| Literature DB >> 26656926 |
Ken'ichi Ichihara1, Chihiro Kohsaka2, Naohiro Tomari2, Tamami Kiyono2, Jun Wada2, Kiyoo Hirooka2, Yoshihiro Yamamoto2.
Abstract
A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.Entities:
Keywords: Fatty acid analysis; Fatty acid methyl ester; Gas chromatography; Triacylglycerol
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Year: 2015 PMID： 26656926 DOI： 10.1016/j.ab.2015.11.009
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365