Helene M Wolsk1, Nilofar V Følsgaard1, Sune Birch1, Susanne Brix2, Trevor T Hansel3, Sebastian L Johnston4, Tatiana Kebadze4, Bo L Chawes1, Klaus Bønnelykke1, Hans Bisgaard1. 1. COPSAC, Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital, University of Copenhagen. 2. Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark. 3. Imperial Clinical Respiratory Research Unit. 4. Airway Disease Infection Section, National Heart and Lung Institute, MRC and Asthma UK Centre in Allergic Mechanisms of Asthma and Centre for Respiratory Infections, Imperial College, London, United Kingdom.
Abstract
BACKGROUND: Bacterial airway colonization is known to alter the airway mucosa immune response in neonates whereas the impact of viruses is unknown. The objective was therefore to examine the effect of respiratory viruses on the immune signature in the airways of asymptomatic neonates. METHODS: Nasal aspirates from 571 asymptomatic 1-month-old neonates from the Copenhagen Prospective Studies on Asthma in Childhood 2010 birth cohort were investigated for respiratory viruses. Simultaneously, unstimulated airway mucosal lining fluid was obtained and quantified for levels of 20 immune mediators related to type 1, type 2, type 17, and regulatory immune paths. The association between immune mediator levels and viruses was tested by conventional statistics and partial least square discriminant analysis. RESULTS: Picornaviruses were detected in 58 neonates (10.2%) and other viruses in 10 (1.8%). A general up-regulation of immune mediators was found in the neonates with picornavirus (P < .0001; partial least square discriminant analysis). The association was pronounced for type 1- and type 2-related markers and was unaffected by comprehensive confounder adjustment. Detection of picornavirus and bacteria was associated with an additive general up-regulating effect. CONCLUSIONS: Asymptomatic presence of picornavirus in the neonatal airway is a potent activator of the topical immune response. This is relevant to understanding the immune potentiating effect of early life exposure to viruses.
BACKGROUND: Bacterial airway colonization is known to alter the airway mucosa immune response in neonates whereas the impact of viruses is unknown. The objective was therefore to examine the effect of respiratory viruses on the immune signature in the airways of asymptomatic neonates. METHODS: Nasal aspirates from 571 asymptomatic 1-month-old neonates from the Copenhagen Prospective Studies on Asthma in Childhood 2010 birth cohort were investigated for respiratory viruses. Simultaneously, unstimulated airway mucosal lining fluid was obtained and quantified for levels of 20 immune mediators related to type 1, type 2, type 17, and regulatory immune paths. The association between immune mediator levels and viruses was tested by conventional statistics and partial least square discriminant analysis. RESULTS: Picornaviruses were detected in 58 neonates (10.2%) and other viruses in 10 (1.8%). A general up-regulation of immune mediators was found in the neonates with picornavirus (P < .0001; partial least square discriminant analysis). The association was pronounced for type 1- and type 2-related markers and was unaffected by comprehensive confounder adjustment. Detection of picornavirus and bacteria was associated with an additive general up-regulating effect. CONCLUSIONS: Asymptomatic presence of picornavirus in the neonatal airway is a potent activator of the topical immune response. This is relevant to understanding the immune potentiating effect of early life exposure to viruses.
Authors: Bo L K Chawes; Matthew J Edwards; Betty Shamji; Christoph Walker; Grant C Nicholson; Andrew J Tan; Nilofar V Følsgaard; Klaus Bønnelykke; Hans Bisgaard; Trevor T Hansel Journal: J Allergy Clin Immunol Date: 2010-03-20 Impact factor: 10.793
Authors: Petra Ina Pfefferle; Gisela Büchele; Nicole Blümer; Marjut Roponen; Markus Johannes Ege; Susanne Krauss-Etschmann; Jon Genuneit; Anne Hyvärinen; Maija-Riitta Hirvonen; Roger Lauener; Juha Pekkanen; Josef Riedler; Jean Charles Dalphin; Bert Brunekeef; Charlotte Braun-Fahrländer; Erika von Mutius; Harald Renz Journal: J Allergy Clin Immunol Date: 2009-12-06 Impact factor: 10.793
Authors: Nilofar V Følsgaard; Susanne Schjørring; Bo L Chawes; Morten A Rasmussen; Karen A Krogfelt; Susanne Brix; Hans Bisgaard Journal: Am J Respir Crit Care Med Date: 2013-01-31 Impact factor: 21.405
Authors: Helene M Wolsk; Bo L Chawes; Jonathan Thorsen; Jakob Stokholm; Klaus Bønnelykke; Susanne Brix; Hans Bisgaard Journal: J Vis Exp Date: 2017-08-07 Impact factor: 1.355
Authors: Beate Zoch-Lesniak; Robert S Ware; Keith Grimwood; Stephen B Lambert Journal: J Pediatric Infect Dis Soc Date: 2020-04-30 Impact factor: 3.164
Authors: Nagarjuna R Cheemarla; Timothy A Watkins; Valia T Mihaylova; Bao Wang; Dejian Zhao; Guilin Wang; Marie L Landry; Ellen F Foxman Journal: medRxiv Date: 2021-01-27
Authors: Nagarjuna R Cheemarla; Timothy A Watkins; Valia T Mihaylova; Bao Wang; Dejian Zhao; Guilin Wang; Marie L Landry; Ellen F Foxman Journal: J Exp Med Date: 2021-06-15 Impact factor: 17.579
Authors: Mohinder Sarna; Robert S Ware; Stephen B Lambert; Theo P Sloots; Michael D Nissen; Keith Grimwood Journal: J Infect Dis Date: 2018-01-17 Impact factor: 5.226