| Literature DB >> 26654901 |
Robert Sertori, Monique Trengove, Faiza Basheer, Alister C Ward, Clifford Liongue.
Abstract
Zebrafish is a powerful model for the study of vertebrate development, being amenable to a wide range of genetic and other manipulations to probe the molecular basis of development and its perturbation in disease. Over recent years, genome editing approaches have become increasingly used as an efficient and sophisticated approach to precisely engineer the zebrafish genome, which has further enhanced the utility of this organism. This review provides a practical overview of genome editing and its application in zebrafish research, including alternate strategies for introducing and screening for specific genetic changes.Entities:
Keywords: CRISPR; HDR; TALENs; genome editing; zebrafish; zinc finger nucleases
Mesh:
Year: 2015 PMID: 26654901 DOI: 10.1093/bfgp/elv051
Source DB: PubMed Journal: Brief Funct Genomics ISSN: 2041-2649 Impact factor: 4.241