Cristina Blanco-Andujar1,2, Daniel Ortega1,3,4, Paul Southern1, Stephen A Nesbitt1, Nguyễn Thị Kim Thanh1,5, Quentin A Pankhurst1. 1. Healthcare Biomagnetics Laboratory, University College London, 21 Albemarle Street, London, W1S 4BS, UK. 2. Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), UMR-7504 CNRS-Université de Strasbourg, 23 rue du Loess, BP 43 67034 Strasbourg Cedex 2, France. 3. Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia), Cantoblanco 28049, Madrid, Spain. 4. Nanobiotecnología (IMDEA-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC), Cantoblanco 28049, Madrid, Spain. 5. Biophysics Group, Department of Physics & Astronomy, University College London, Gower Street, London, WC1E 6BT, UK.
Abstract
AIM: To assess cell death pathways in response to magnetic hyperthermia. MATERIALS & METHODS: Human melanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis. RESULTS: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature. CONCLUSION: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.
AIM: To assess cell death pathways in response to magnetic hyperthermia. MATERIALS & METHODS:Humanmelanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis. RESULTS: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature. CONCLUSION: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.
Entities:
Keywords:
apoptosis; cell death pathways; human melanoma cells; magnetic hyperthermia; magnetic nanoparticles
Authors: Julian Palzer; Benedikt Mues; Richard Goerg; Merel Aberle; Sander S Rensen; Steven W M Olde Damink; Rianne D W Vaes; Thorsten Cramer; Thomas Schmitz-Rode; Ulf P Neumann; Ioana Slabu; Anjali A Roeth Journal: Int J Nanomedicine Date: 2021-04-23
Authors: Tammy L Kalber; Katherine L Ordidge; Paul Southern; Michael R Loebinger; Panagiotis G Kyrtatos; Quentin A Pankhurst; Mark F Lythgoe; Sam M Janes Journal: Int J Nanomedicine Date: 2016-05-09
Authors: Ulrich M Engelmann; Anjali A Roeth; Dietmar Eberbeck; Eva M Buhl; Ulf P Neumann; Thomas Schmitz-Rode; Ioana Slabu Journal: Sci Rep Date: 2018-09-04 Impact factor: 4.379