Li-Chu Tsai1, Hao-Ying Hsieh1,2, Kun-Ying Lu3, Sin-Yu Wang3, Fwu-Long Mi3,4,5. 1. Institute of Organic & Polymeric Materials, National Taipei University of Technology, Taipei 10608, Taiwan. 2. Institute of Biomedical Engineering, National Taiwan University, Taipei 10002, Taiwan. 3. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan. 4. Department of Biochemistry & Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan. 5. Graduate Institute of Nanomedicine & Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
Abstract
AIM: Development of epigallocatechin gallate (EGCG) and gelatin-doxorubicin conjugate (GLT-DOX)-coated gold nanoparticles (DOX-GLT/EGCG AuNPs) for fluorescence imaging and inhibition of prostate cancer cell growth. MATERIALS & METHODS: AuNPs alternatively coated with EGCG and DOX-GLT conjugates were prepared by a layer-by-layer assembly method. The physicochemical properties of the AuNPs and the effect of Laminin 67R receptor-mediated endocytosis on the anticancer efficacy of the AuNPs were examined. RESULTS: The AuNPs significantly inhibit the proliferation of PC-3 cancer cell and the enzyme-responsive intracellular release of DOX could be tracked by monitoring the recovery of the fluorescence signal of DOX. CONCLUSION: Laminin 67R receptor-mediated delivery of DOX using the AuNPs enhanced cellular uptake of DOX and improved apoptosis of PC-3 cells.
AIM: Development of epigallocatechin gallate (EGCG) and gelatin-doxorubicin conjugate (GLT-DOX)-coated gold nanoparticles (DOX-GLT/EGCG AuNPs) for fluorescence imaging and inhibition of prostate cancer cell growth. MATERIALS & METHODS: AuNPs alternatively coated with EGCG and DOX-GLT conjugates were prepared by a layer-by-layer assembly method. The physicochemical properties of the AuNPs and the effect of Laminin 67R receptor-mediated endocytosis on the anticancer efficacy of the AuNPs were examined. RESULTS: The AuNPs significantly inhibit the proliferation of PC-3 cancer cell and the enzyme-responsive intracellular release of DOX could be tracked by monitoring the recovery of the fluorescence signal of DOX. CONCLUSION: Laminin 67R receptor-mediated delivery of DOX using the AuNPs enhanced cellular uptake of DOX and improved apoptosis of PC-3 cells.
Entities:
Keywords:
EGCG; Lam 67R; MMP-2; cellular uptake; doxorubicin; drug release; gelatin; gold nanoparticles; prostate cancer
Authors: Charles Samuel Umbaugh; Adriana Diaz-Quiñones; Manoel Figueiredo Neto; Joseph J Shearer; Marxa L Figueiredo Journal: Oncotarget Date: 2017-12-13