Anna M Bogdali, Bogdali M Anna1, Antoszczyk Grazyna2, Dyga Wojciech3, Obtulowicz Aleksander4, Bialecka Anna5, Kasprowicz Andrzej6, Magnowska Zofia7, Obtulowicz Krystyna8. 1. Department of Clinical and Environmental Allergology, JagiellonianUniversity Medical College, 31-531 Krakow, Poland. Electronic address: am.bogdali@uj.edu.pl. 2. Department of Clinical and Environmental Allergology, JagiellonianUniversity Medical College, 31-531 Krakow, Poland. Electronic address: grazyna.antoszczyk@gmail.com. 3. Department of Clinical and Environmental Allergology, JagiellonianUniversity Medical College, 31-531 Krakow, Poland. Electronic address: wdyga@cm-uj.krakow.pl. 4. Department of Dermatology, Jagiellonian University Medical College, 31-066 Krakow, Poland. Electronic address: obtulowicz@poczta.fm. 5. Centre for Microbiological Research and Autovaccines Ltd., 31-016 Krakow, Poland. Electronic address: bialecka.ania@gmail.com. 6. Centre for Microbiological Research and Autovaccines Ltd., 31-016 Krakow, Poland. Electronic address: a.kasprowicz@cbm.com.pl. 7. University of Copenhagen Research Centre for Control of Antibiotics Resistance-UC-Care Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870 Frb. C., 1-20 Frb. C., Copenhagen, Denmark. Electronic address: zofia@sund.ku. 8. Department of Clinical and Environmental Allergology, JagiellonianUniversity Medical College, 31-531 Krakow, Poland.
Abstract
BACKGROUND: The increase of nickel air pollution is supposed to frequent side effects of nickel action related to virulence potential of Staphylococcus aureus in patients with nickel allergy in atopic dermatitis. The goal was to investigate the relationship between nickel allergy and infection by S. aureus in atopic dermatitis. METHODS: Nickel allergy was confirmed in atopic patients and excluded in healthy volunteers using patch testing. Infection by S. aureus was tested in atopic patients and healthy volunteers by use of API Staph system. The specific IgE for staphylococcal enterotoxin A and B were measured. Secretion of IFN-g, IL-2, IL-13 by PBMC under nickel sulfate and the enterotoxins A and B stimulations were studied with ELISpot. RESULTS: We found the increased number of infections by S. aureus in atopic patients with nickel allergy in comparison to atopic patients and healthy volunteers without nickel allergy. The elevated secretion of IL-2 under nickel sulfate stimulation in vitro was exclusively found in atopic patients with nickel allergy infected by S. aureus. CONCLUSIONS: Our data suggest that nickel allergy and infection by S. aureus are linked in atopic dermatitis.
BACKGROUND: The increase of nickel air pollution is supposed to frequent side effects of nickel action related to virulence potential of Staphylococcus aureus in patients with nickelallergy in atopic dermatitis. The goal was to investigate the relationship between nickelallergy and infection by S. aureus in atopic dermatitis. METHODS:Nickelallergy was confirmed in atopic patients and excluded in healthy volunteers using patch testing. Infection by S. aureus was tested in atopic patients and healthy volunteers by use of API Staph system. The specific IgE for staphylococcal enterotoxin A and B were measured. Secretion of IFN-g, IL-2, IL-13 by PBMC under nickel sulfate and the enterotoxins A and B stimulations were studied with ELISpot. RESULTS: We found the increased number of infections by S. aureus in atopic patients with nickelallergy in comparison to atopic patients and healthy volunteers without nickelallergy. The elevated secretion of IL-2 under nickel sulfate stimulation in vitro was exclusively found in atopic patients with nickelallergy infected by S. aureus. CONCLUSIONS: Our data suggest that nickelallergy and infection by S. aureus are linked in atopic dermatitis.