Talita Gnoato Zotz1, Luiz Guilherme A Capriglione2, Rafael Zotz3, Lucia Noronha4, Marina Louise Viola De Azevedo5, Hilana Rickli Fiuza Martins6, Anna Raquel Silveira Gomes7. 1. Physiotherapy Department, Pontifical Catholic University of Parana, Curitiba, Paraná, Brazil. Electronic address: talita.gnoato@gmail.com. 2. Health Science Program, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. 3. Vivarium Department, Pontifical Catholic University of Parana, Curitiba, Paraná, Brazil. 4. School of Medicine - Experimental Pathology Laboratory, Masters and Doctorate Programs in Health Science of the Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. 5. Experimental Pathology Department, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. 6. Physical Education Department, Federal University of Paraná, Curitiba, Paraná, Brazil. 7. Physiotherapy Department, Masters and Doctorate Programs in Physical Education, Federal University of Paraná, Curitiba, Paraná, Brazil.
Abstract
UNLABELLED: It has been shown that stretching exercises can improve the flexibility and independence of the elderly. However, although these exercises commonly constitute training programs, the morphological adaptations induced by stretching exercises in aged skeletal muscle are still unclear. OBJECTIVE: To assess the acute effects of passive mechanical static stretching on the morphology, sarcomerogenesis and modulation of important components of the extracellular matrix of the soleus muscle of aged female rats. METHODS: Fifteen old female rats with 26 months were divided into two groups: stretching (n=8, SG) and control (n=7, CG): The stretching protocol consisted of 4 repetitions each of 1 min with 30s interval between sets. Stretching was performed on the left soleus muscle, 3 times a week for 1 week. After three sessions, the rats were anesthetized to remove the left soleus muscle, and then euthanized. The following analyses were carried out: muscle fiber cross-sectional area and serial sarcomere number; immunohistochemistry for the quantification of collagen I, III and TGFβ-1. RESULTS: a decrease in muscle fiber cross-sectional area of the SG was observed when compared to the CG (p=0.0001, Kruskal-Wallis); the percentage of type I collagen was significantly lower in the SG when compared to the CG (p=0.01, Kruskal-Wallis), as well as the percentage of TGFβ-1 (p=0.04, Kruskal-Wallis); collagen III was significantly higher in the SG than in the CG (7.06±6.88% vs 4.92±5.30%, p=0.01, Kruskal-Wallis). CONCLUSION: Although the acute stretching induced muscle hypotrophy, an antifibrotic action was detected.
UNLABELLED: It has been shown that stretching exercises can improve the flexibility and independence of the elderly. However, although these exercises commonly constitute training programs, the morphological adaptations induced by stretching exercises in aged skeletal muscle are still unclear. OBJECTIVE: To assess the acute effects of passive mechanical static stretching on the morphology, sarcomerogenesis and modulation of important components of the extracellular matrix of the soleus muscle of aged female rats. METHODS: Fifteen old female rats with 26 months were divided into two groups: stretching (n=8, SG) and control (n=7, CG): The stretching protocol consisted of 4 repetitions each of 1 min with 30s interval between sets. Stretching was performed on the left soleus muscle, 3 times a week for 1 week. After three sessions, the rats were anesthetized to remove the left soleus muscle, and then euthanized. The following analyses were carried out: muscle fiber cross-sectional area and serial sarcomere number; immunohistochemistry for the quantification of collagen I, III and TGFβ-1. RESULTS: a decrease in muscle fiber cross-sectional area of the SG was observed when compared to the CG (p=0.0001, Kruskal-Wallis); the percentage of type I collagen was significantly lower in the SG when compared to the CG (p=0.01, Kruskal-Wallis), as well as the percentage of TGFβ-1 (p=0.04, Kruskal-Wallis); collagen III was significantly higher in the SG than in the CG (7.06±6.88% vs 4.92±5.30%, p=0.01, Kruskal-Wallis). CONCLUSION: Although the acute stretching induced muscle hypotrophy, an antifibrotic action was detected.
Authors: Ivo V de Sousa Neto; João L Q Durigan; Vinicius Guzzoni; Ramires A Tibana; Jonato Prestes; Heloisa S Selistre de Araujo; Rita de Cássia Marqueti Journal: Front Physiol Date: 2018-03-12 Impact factor: 4.566
Authors: Sabrina M Peviani; Vinicius Guzzoni; Clara M Pinheiro-Dardis; Yara P da Silva; Alisson C R Fioravante; Adriana H Sagawa; Gabriel B Delfino; João L Q Durigan; Tania F Salvini Journal: Sci Rep Date: 2018-06-13 Impact factor: 4.379