Keila Karine Duarte Campos1, Simone Floresta Leal2, Daniela Caldeira Costa3, Wanderson Geraldo de Lima4, Frank Silva Bezerra1. 1. a Laboratory of Experimental Pathophysiology (LAFEX), Department of Biological Sciences (DECBI) , Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP) , Ouro Preto, MG, Brazil. 2. b Graduating in Medicine, School of Medicine , Federal University of Ouro Preto (UFOP) , Ouro Preto, MG, Brazil. 3. c Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB) , Federal University of Ouro Preto (UFOP) , Ouro Preto, MG, Brazil. 4. d Laboratory of Morphopathology (LMP), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB) , Federal University of Ouro Preto (UFOP) , Ouro Preto , MG , Brazil.
Abstract
PURPOSE: The aim of this study was to evaluate of the effect of distilled water and saline ultrasonic nebulization on the inflammatory and oxidative stress responses and on the lower airway architecture. MATERIALS AND METHODS: Twenty-one male Fischer rats were distributed into 3 groups of 7 animals each: a control group (CG), exposed to ambient air; a saline group (SG), exposed to 0.9% sodium chloride (NaCl); and a group exposed to distilled water (DWG). The exposure was carried out in a box attached to an ultrasonic inhaler, occurring for 20 min, 3 times a day for 6 months. At 24h after the last exposure, the animals were euthanized. The bronchoalveolar lavage fluid (BALF) and lungs were collected for study. RESULTS: There was an increase of inflammatory cells in the pulmonary tissue BALF in the DWG compared with the CG. The DWG showed an increase of inflammatory cells compared with the SG and CG. The DWG and SG had higher NADPH oxidase activity than the CG. The volume density (Vv) of the alveolar septum was higher in the DWG than in the SG and CG, and the DWG also had a higher Vv of collagen fibers than the other 2 groups. The DWG presented elevated content of thiobarbituric acid reactive substances in lung homogenates relative to the SG and CG. CONCLUSIONS: The ultrasonic nebulization of distilled water increased the influx of inflammatory cells and oxidative damage, and promoted changes in the lung architecture.
PURPOSE: The aim of this study was to evaluate of the effect of distilled water and saline ultrasonic nebulization on the inflammatory and oxidative stress responses and on the lower airway architecture. MATERIALS AND METHODS: Twenty-one male Fischer rats were distributed into 3 groups of 7 animals each: a control group (CG), exposed to ambient air; a saline group (SG), exposed to 0.9% sodium chloride (NaCl); and a group exposed to distilled water (DWG). The exposure was carried out in a box attached to an ultrasonic inhaler, occurring for 20 min, 3 times a day for 6 months. At 24h after the last exposure, the animals were euthanized. The bronchoalveolar lavage fluid (BALF) and lungs were collected for study. RESULTS: There was an increase of inflammatory cells in the pulmonary tissue BALF in the DWG compared with the CG. The DWG showed an increase of inflammatory cells compared with the SG and CG. The DWG and SG had higher NADPH oxidase activity than the CG. The volume density (Vv) of the alveolar septum was higher in the DWG than in the SG and CG, and the DWG also had a higher Vv of collagen fibers than the other 2 groups. The DWG presented elevated content of thiobarbituric acid reactive substances in lung homogenates relative to the SG and CG. CONCLUSIONS: The ultrasonic nebulization of distilled water increased the influx of inflammatory cells and oxidative damage, and promoted changes in the lung architecture.
Authors: Karina Braga Pena; Camila de Oliveira Ramos; Nícia Pedreira Soares; Pamela Félix da Silva; Ana Carla Balthar Bandeira; Guilherme de Paula Costa; Sílvia Dantas Cangussú; André Talvani; Frank Silva Bezerra Journal: Int J Chron Obstruct Pulmon Dis Date: 2016-12-15
Authors: Daniela Fonseca Abdo Rocha; Pedro Alves Machado-Junior; Ana Beatriz Farias Souza; Thalles de Freitas Castro; Guilherme de Paula Costa; André Talvani; Frank Silva Bezerra; Silvia Dantas Cangussú Journal: Biomed Res Int Date: 2021-11-25 Impact factor: 3.411
Authors: Ana Beatriz Farias de Souza; Máira Tereza Talma Chírico; Christiane Teixeira Cartelle; Guilherme de Paula Costa; André Talvani; Sílvia Dantas Cangussú; Rodrigo Cunha Alvim de Menezes; Frank Silva Bezerra Journal: Oxid Med Cell Longev Date: 2018-02-12 Impact factor: 6.543