Eleonora Longhin1, Laura Capasso1, Cristina Battaglia2, Maria Carla Proverbio3, Cristina Cosentino4, Ingrid Cifola3, Eleonora Mangano5, Marina Camatini1, Maurizio Gualtieri6. 1. Department of Earth and Environmental Sciences, POLARIS Research Centre, University of Milano-Bicocca, Piazza della Scienza 1, Milano 20126, Italy. 2. Department of medical biotechnology and translational medicine (BIOMETRA), Università degli Studi di Milano, 93 via F.lli Cervi, Segrate 20900, Italy; Institute of biomedical technology, CNR, Via F.lli Cervi, Segrate 20900, Italy. Electronic address: cristina.battaglia@unimi.it. 3. Department of Physiopathology and Transplantation, Università degli Studi di Milano, 20090, Italy. 4. Department of medical biotechnology and translational medicine (BIOMETRA), Università degli Studi di Milano, 93 via F.lli Cervi, Segrate 20900, Italy. 5. Institute of biomedical technology, CNR, Via F.lli Cervi, Segrate 20900, Italy. 6. ENEA-UTTS/SSPT-MET-INAT, Strada per Crescentino 41, 13040, Saluggia (VC), Italy. Electronic address: maurizio.gualtieri@univ-littoral.fr.
Abstract
BACKGROUND: Exposure to particulate matter (PM) is associated with various health effects. Physico-chemical properties influence the toxicological impact of PM, nonetheless the mechanisms underlying PM-induced effects are not completely understood. OBJECTIVES: Human bronchial epithelial cells were used to analyse the pathways activated after exposure to summer and winter urban PM and to identify possible markers of exposure. METHODS: BEAS-2B cells were exposed for 24 h to 10 μg/cm(2) of winter PM2.5 (wPM) and summer PM10 (sPM) sampled in Milan. A microarray technology was used to profile the cells gene expression. Genes and microRNAs were analyzed by bioinformatics technique to identify pathways involved in cellular responses. Selected genes and pathways were validated at protein level (western blot, membrane protein arrays and ELISA). RESULTS: The molecular networks activated by the two PM evidenced a correlation among oxidative stress, inflammation and DNA damage responses. sPM induced the release of pro-inflammatory mediators, although miR-146a and genes related to inflammation resulted up-regulated by both PM. Moreover both PM affected a set of genes, proteins and miRNAs related to antioxidant responses, cancer development, extracellular matrix remodeling and cytoskeleton organization, while miR-29c, implicated in epigenetic modification, resulted up-regulated only by wPM. sPM effects may be related to biological and inorganic components, while wPM apparently related to the high content of organic compounds. CONCLUSIONS: These results may be helpful for the individuation of biomarkers for PM exposure, linked to the specific PM physico-chemical properties.
BACKGROUND: Exposure to particulate matter (PM) is associated with various health effects. Physico-chemical properties influence the toxicological impact of PM, nonetheless the mechanisms underlying PM-induced effects are not completely understood. OBJECTIVES:Human bronchial epithelial cells were used to analyse the pathways activated after exposure to summer and winter urban PM and to identify possible markers of exposure. METHODS: BEAS-2B cells were exposed for 24 h to 10 μg/cm(2) of winter PM2.5 (wPM) and summer PM10 (sPM) sampled in Milan. A microarray technology was used to profile the cells gene expression. Genes and microRNAs were analyzed by bioinformatics technique to identify pathways involved in cellular responses. Selected genes and pathways were validated at protein level (western blot, membrane protein arrays and ELISA). RESULTS: The molecular networks activated by the two PM evidenced a correlation among oxidative stress, inflammation and DNA damage responses. sPM induced the release of pro-inflammatory mediators, although miR-146a and genes related to inflammation resulted up-regulated by both PM. Moreover both PM affected a set of genes, proteins and miRNAs related to antioxidant responses, cancer development, extracellular matrix remodeling and cytoskeleton organization, while miR-29c, implicated in epigenetic modification, resulted up-regulated only by wPM. sPM effects may be related to biological and inorganic components, while wPM apparently related to the high content of organic compounds. CONCLUSIONS: These results may be helpful for the individuation of biomarkers for PM exposure, linked to the specific PM physico-chemical properties.
Authors: Helena Libalova; Pavel Rossner; Kristyna Vrbova; Tana Brzicova; Jitka Sikorova; Michal Vojtisek-Lom; Vit Beranek; Jiri Klema; Miroslav Ciganek; Jiri Neca; Katerina Pencikova; Miroslav Machala; Jan Topinka Journal: Int J Mol Sci Date: 2016-11-03 Impact factor: 5.923
Authors: Juan C Gonzalez-Rivera; Kevin C Baldridge; Dongyu S Wang; Kanan Patel; Jamie C L Chuvalo-Abraham; Lea Hildebrandt Ruiz; Lydia M Contreras Journal: Commun Biol Date: 2020-07-22
Authors: Iselin Rynning; Jiri Neca; Kristyna Vrbova; Helena Libalova; Pavel Rossner; Jørn A Holme; Kristine B Gützkow; Anani K Johnny Afanou; Yke J Arnoldussen; Eva Hruba; Øivind Skare; Aage Haugen; Jan Topinka; Miroslav Machala; Steen Mollerup Journal: Toxicol Sci Date: 2018-11-01 Impact factor: 4.849