Michele Vacca1, Marco Di Eusanio2, Marica Cariello3, Giusi Graziano3, Simona D'Amore4, Francesco Dimitri Petridis2, Andria D'orazio5, Lorena Salvatore5, Antonio Tamburro5, Gianluca Folesani2, David Rutigliano6, Fabio Pellegrini5, Carlo Sabbà7, Giuseppe Palasciano7, Roberto Di Bartolomeo2, Antonio Moschetta8. 1. Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy Ageing Research Center (CeSI), 'G. d'Annunzio' University Foundation, Chieti, Italy. 2. Cardiovascular Department, 'S.Orsola Malpighi' Hospital, University of Bologna, Bologna, Italy. 3. Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy National Cancer Research Center IRCCS 'Giovanni Paolo II', Bari, Italy. 4. Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy National Cancer Research Center IRCCS 'Giovanni Paolo II', Bari, Italy. 5. Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy. 6. Unit of Cardiology, 'San Paolo' Hospital, Bari, Italy. 7. Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy. 8. Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', Piazza Giulio Cesare 11, 70124 Bari, Italy Fondazione Mario Negri Sud, Santa Maria Imbaro (CH), Italy National Cancer Research Center IRCCS 'Giovanni Paolo II', Bari, Italy antonio.moschetta@uniba.it.
Abstract
BACKGROUND: Epicardial adipose tissue (EAT) is an atypical fat depot surrounding the heart with a putative role in the development of atherosclerosis. METHODS AND RESULTS: We profiled genes and miRNAs in perivascular EAT and subcutaneous adipose tissue (SAT) of metabolically healthy patients without coronary artery disease (CAD) vs. metabolic patients with CAD. Compared with SAT, a specific tuning of miRNAs and genes points to EAT as a tissue characterized by a metabolically active and pro-inflammatory profile. Then, we depicted both miRNA and gene signatures of EAT in CAD, featuring a down-regulation of genes involved in lipid metabolism, mitochondrial function, nuclear receptor transcriptional activity, and an up-regulation of those involved in antigen presentation, chemokine signalling, and inflammation. Finally, we identified miR-103-3p as candidate modulator of CCL13 in EAT, and a potential biomarker role for the chemokine CCL13 in CAD. CONCLUSION: EAT in CAD is characterized by changes in the regulation of metabolism and inflammation with miR-103-3p/CCL13 pair as novel putative actors in EAT function and CAD. Published on behalf of the European Society of Cardiology. All rights reserved.
BACKGROUND: Epicardial adipose tissue (EAT) is an atypical fat depot surrounding the heart with a putative role in the development of atherosclerosis. METHODS AND RESULTS: We profiled genes and miRNAs in perivascular EAT and subcutaneous adipose tissue (SAT) of metabolically healthy patients without coronary artery disease (CAD) vs. metabolic patients with CAD. Compared with SAT, a specific tuning of miRNAs and genes points to EAT as a tissue characterized by a metabolically active and pro-inflammatory profile. Then, we depicted both miRNA and gene signatures of EAT in CAD, featuring a down-regulation of genes involved in lipid metabolism, mitochondrial function, nuclear receptor transcriptional activity, and an up-regulation of those involved in antigen presentation, chemokine signalling, and inflammation. Finally, we identified miR-103-3p as candidate modulator of CCL13 in EAT, and a potential biomarker role for the chemokine CCL13 in CAD. CONCLUSION: EAT in CAD is characterized by changes in the regulation of metabolism and inflammation with miR-103-3p/CCL13 pair as novel putative actors in EAT function and CAD. Published on behalf of the European Society of Cardiology. All rights reserved.
Authors: Kanta Chechi; Jinchu Vijay; Pierre Voisine; Patrick Mathieu; Yohan Bossé; Andre Tchernof; Elin Grundberg; Denis Richard Journal: JCI Insight Date: 2019-04-18
Authors: João Adriano Sousa; Maria Isabel Mendonça; Marco Serrão; Sofia Borges; Eva Henriques; Sónia Freitas; Margarida Tentem; Marina Santos; Pedro Freitas; António Ferreira; Graça Guerra; António Drumond; Roberto Palma Reis Journal: Clin Med Insights Cardiol Date: 2021-07-03