Literature DB >> 26640034

Site-Specific Polymer Conjugation Stabilizes Therapeutic Enzymes in the Gastrointestinal Tract.

Jessica D Schulz1, Melanie Patt1, Sophie Basler1, Hajo Kries2, Donald Hilvert2, Marc A Gauthier3, Jean-Christophe Leroux1.   

Abstract

The site-specific conjugation of polymers to multiple engineered cysteine residues of a prolyl endopeptidase leads to its stabilization in the gastrointestinal tract of rats, without compromising the activity relative to the native enzyme. The importance of polymer attachment sites is investigated, as well as the significance of polymer structure.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  gastrointestinal stability; poly(ethylene glycol); site-specific polymer conjugation, therapeutic enzymes

Mesh：

Substances：

Year:  2015        PMID： 26640034     DOI： 10.1002/adma.201504797

Source DB:  PubMed          Journal:  Adv Mater        ISSN： 0935-9648            Impact factor:   30.849


  6 in total

1.  A Modular Method for the High-Yield Synthesis of Site-Specific Protein-Polymer Therapeutics.

Authors:  Yan Pang; Jinyao Liu; Yizhi Qi; Xinghai Li; Ashutosh Chilkoti
Journal:  Angew Chem Int Ed Engl       Date:  2016-07-21       Impact factor: 15.336

Review 2.  Application of Fluorescence in Studying Therapeutic Enzymes.

Authors:  Zhaoshuai Wang; Caihong Li; Yinan Wei
Journal:  Adv Exp Med Biol       Date:  2019       Impact factor: 2.622

Review 3.  Engineering Strategies for Oral Therapeutic Enzymes to Enhance Their Stability and Activity.

Authors:  Philipp Lapuhs; Gregor Fuhrmann
Journal:  Adv Exp Med Biol       Date:  2019       Impact factor: 2.622

4.  An MRI-guided HIFU-triggered wax-coated capsule for supertargeted drug release: a proof-of-concept study.

Authors:  Simon Matoori; Maurizio Roveri; Peter Tiefenboeck; Annatina Romagna; Olha Wuerthinger; Orpheus Kolokythas; Johannes M Froehlich
Journal:  Eur Radiol Exp       Date:  2019-03-05

5.  A microparticulate based formulation to protect therapeutic enzymes from proteolytic digestion: phenylalanine ammonia lyase as case study.

Authors:  Irene Pereira de Sousa; Charlotte Gourmel; Olena Berkovska; Michael Burger; Jean-Christophe Leroux
Journal:  Sci Rep       Date:  2020-02-27       Impact factor: 4.379

6.  Transforming protein-polymer conjugate purification by tuning protein solubility.

Authors:  Stefanie L Baker; Aravinda Munasinghe; Bibifatima Kaupbayeva; Nin Rebecca Kang; Marie Certiat; Hironobu Murata; Krzysztof Matyjaszewski; Ping Lin; Coray M Colina; Alan J Russell
Journal:  Nat Commun       Date:  2019-10-17       Impact factor: 14.919
  6 in total

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