Yul-Lye Hwang1, Myung Im2, Min-Ho Lee3, Seok-Seon Roh4, Byoung Wook Choi5, Sue Jeong Kim2, Kyung-Cheol Sohn2, Young Lee2, Young-Joon Seo2, Jeung-Hoon Lee6, Chang Deok Kim7. 1. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea; Oriental BioMed Lab, Daejeon, Republic of Korea. 2. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea. 3. Oriental BioMed Lab, Daejeon, Republic of Korea. 4. Oriental Medical College of Daejeon University, Daejeon, Republic of Korea. 5. Department of Chemical & Biological Engineering, Hanbat National University, Daejeon, Republic of Korea. 6. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea; Skin Med Company, Daejeon, Republic of Korea. 7. Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea. Electronic address: cdkimd@cnu.ac.kr.
Abstract
AIMS: Acne is a common skin disease that originates in the sebaceous gland. The pathogenesis of acne is very complex, involving the increase of sebum production and perifollicular inflammation. In this study, we screened the anti-lipogenic material and demonstrated its effect using cultured human sebocytes. MAIN METHODS: Normal human sebocytes were cultured by explanting the sebaceous glands. To evaluate the anti-lipogenic effect, sebocytes were treated with test materials and (14)C-acetate incorporation assay was performed. KEY FINDINGS: To screen the anti-lipogenic materials, we tested the effect of many herbal plant extracts. We found that Angelica dahurica extract inhibited the insulin-like growth factor-1 (IGF-1)-induced sebum production in terms of squalene synthesis in sebocytes. Furthermore, imperatorin isolated from A. dahurica showed remarkable inhibitory effect on squalene production as well as squalene synthase promoter activity. To investigate the putative action mechanism, we tested the effect of imperatorin on intracellular signaling. The results showed that imperatorin inhibited IGF-1-induced phosphorylation of Akt. In addition, imperatorin significantly down-regulated PPAR-γ and SREBP-1, the important transcription factors for lipid synthesis. SIGNIFICANCE: These results suggest that imperatorin has a potential for reducing sebum production in sebocytes, and can be applicable for acne treatment.
AIMS: Acne is a common skin disease that originates in the sebaceous gland. The pathogenesis of acne is very complex, involving the increase of sebum production and perifollicular inflammation. In this study, we screened the anti-lipogenic material and demonstrated its effect using cultured human sebocytes. MAIN METHODS: Normal human sebocytes were cultured by explanting the sebaceous glands. To evaluate the anti-lipogenic effect, sebocytes were treated with test materials and (14)C-acetate incorporation assay was performed. KEY FINDINGS: To screen the anti-lipogenic materials, we tested the effect of many herbal plant extracts. We found that Angelica dahurica extract inhibited the insulin-like growth factor-1 (IGF-1)-induced sebum production in terms of squalene synthesis in sebocytes. Furthermore, imperatorin isolated from A. dahurica showed remarkable inhibitory effect on squalene production as well as squalene synthase promoter activity. To investigate the putative action mechanism, we tested the effect of imperatorin on intracellular signaling. The results showed that imperatorin inhibited IGF-1-induced phosphorylation of Akt. In addition, imperatorin significantly down-regulated PPAR-γ and SREBP-1, the important transcription factors for lipid synthesis. SIGNIFICANCE: These results suggest that imperatorin has a potential for reducing sebum production in sebocytes, and can be applicable for acne treatment.
Authors: Cong Wang; Yul-Lye Hwang; Xue Mei Li; Soo Jung Kim; Ming Ji Zhu; Jeung-Hoon Lee; Ri-Hua Jiang; Chang Deok Kim Journal: Ann Dermatol Date: 2019-05-01 Impact factor: 1.444