Hui Dong1, Cheng Wang1,2, Sunbin Lu1, Cuiyu Yu3, Lei Huang1, Wuruo Feng3, Hui Xu3, Xi Chen1, Ke Zen1, Qiao Yan1, Weiguo Liu3, Chunni Zhang1,2, Chen-Yu Zhang1. 1. a State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences, Nanjing University School of Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing University , Nanjing , China . 2. b Department of Clinical Laboratory , Jinling Hospital, Nanjing University School of Medicine , Nanjing , China , and. 3. c Department of Neurology , Affiliated Brain Hospital of Nanjing Medical University , Nanjing , China.
Abstract
CONTEXT: Sensitive, non-invasive biomarkers that facilitate Parkinson's disease (PD) detection and stage assignment are currently unavailable. OBJECTIVE: The objective of this study is to investigate the potential of circulating microRNAs (miRNAs) as novel biomarkers for PD. MATERIALS AND METHODS: Solexa sequencing technology and quantitative real-time PCR were applied to screen and verify altered serum miRNAs in PD patients. RESULTS: Serum miR-141, miR-214, miR-146b-5p, and miR-193a-3p were decreased significantly in PD patients compared with controls. Furthermore, the 4-miRNA panel enabled the differentiation of HY stage 1 and 2 PD patients from controls. DISCUSSION AND CONCLUSION: The four serum miRNAs may represent novel biomarkers for the early detection of PD.
CONTEXT: Sensitive, non-invasive biomarkers that facilitate Parkinson's disease (PD) detection and stage assignment are currently unavailable. OBJECTIVE: The objective of this study is to investigate the potential of circulating microRNAs (miRNAs) as novel biomarkers for PD. MATERIALS AND METHODS: Solexa sequencing technology and quantitative real-time PCR were applied to screen and verify altered serum miRNAs in PDpatients. RESULTS: Serum miR-141, miR-214, miR-146b-5p, and miR-193a-3p were decreased significantly in PDpatients compared with controls. Furthermore, the 4-miRNA panel enabled the differentiation of HY stage 1 and 2 PDpatients from controls. DISCUSSION AND CONCLUSION: The four serum miRNAs may represent novel biomarkers for the early detection of PD.
Authors: Marcelo A Mori; Raissa G Ludwig; Ruben Garcia-Martin; Bruna B Brandão; C Ronald Kahn Journal: Cell Metab Date: 2019-08-22 Impact factor: 27.287