Paola Valbonesi1,2, Silvia Franzellitti1,2, Ferdinando Bersani3, Andrea Contin1,3, Elena Fabbri1,2. 1. a Interdepartmental Centre for Environmental Science Research, University of Bologna , Campus of Ravenna , Italy ; 2. b Department of Biological, Geological and Environmental Sciences , University of Bologna , Bologna , Italy ; 3. c Department of Physics , University of Bologna , Bologna , Italy.
Abstract
PURPOSE: Due to its role in learning, memory and in many neurodegenerative diseases, acetylcholinesterase (AChE) represents an interesting endpoint to assess possible targets of exposure to radiofrequency electromagnetic fields (RF-EMF) generated by mobile phones. We investigated possible alterations of enzymatic activity, gene and protein expression of AChE in neuronal-like cells exposed to a 1.8 GHz Global System for Mobile Communication (GSM) modulated signal (217-GSM). MATERIALS AND METHODS: Rat PC12 cells were exposed for 24 h to 1.8 GHz 217-GSM signal. Specific adsorption rate (SAR) was 2 W/kg. AChE enzyme activity was assessed spectrophotometrically by Ellman's method, mRNA expression level was evaluated by real time polymerase chain reaction, and protein expression was assessed by Western blotting. RESULTS: AChE enzymatic activity increased of 1.4-fold in PC12 cells exposed to 217-GSM signal for 24 h, whilst AChE transcriptional or translational pathways were not affected. CONCLUSION: Our results provide the first evidence of effects on AChE activity after in vitro exposure of mammalian cells to the RF-EMF generated by GSM mobile phones, at the SAR value 2 W/kg. The obtained evidence promotes further investigations on AChE as a possible target of RF-EMF and confirm the ability of 1.8 GHz 217-GSM signal to induce biological effects in different mammalian cells.
PURPOSE: Due to its role in learning, memory and in many neurodegenerative diseases, acetylcholinesterase (AChE) represents an interesting endpoint to assess possible targets of exposure to radiofrequency electromagnetic fields (RF-EMF) generated by mobile phones. We investigated possible alterations of enzymatic activity, gene and protein expression of AChE in neuronal-like cells exposed to a 1.8 GHz Global System for Mobile Communication (GSM) modulated signal (217-GSM). MATERIALS AND METHODS:Rat PC12 cells were exposed for 24 h to 1.8 GHz 217-GSM signal. Specific adsorption rate (SAR) was 2 W/kg. AChE enzyme activity was assessed spectrophotometrically by Ellman's method, mRNA expression level was evaluated by real time polymerase chain reaction, and protein expression was assessed by Western blotting. RESULTS:AChE enzymatic activity increased of 1.4-fold in PC12 cells exposed to 217-GSM signal for 24 h, whilst AChE transcriptional or translational pathways were not affected. CONCLUSION: Our results provide the first evidence of effects on AChE activity after in vitro exposure of mammalian cells to the RF-EMF generated by GSM mobile phones, at the SAR value 2 W/kg. The obtained evidence promotes further investigations on AChE as a possible target of RF-EMF and confirm the ability of 1.8 GHz 217-GSM signal to induce biological effects in different mammalian cells.
Entities:
Keywords:
217-GSM signal; acetylcholinesterase activity; acetylcholinesterase gene and protein expression
Authors: Palalle G Tharushi Perera; The Hong Phong Nguyen; Chaitali Dekiwadia; Jason V Wandiyanto; Igor Sbarski; Olga Bazaka; Kateryna Bazaka; Russell J Crawford; Rodney J Croft; Elena P Ivanova Journal: Int J Nanomedicine Date: 2018-12-10
Authors: Palalle G Tharushi Perera; Dominique R T Appadoo; Samuel Cheeseman; Jason V Wandiyanto; Denver Linklater; Chaitali Dekiwadia; Vi Khanh Truong; Mark J Tobin; Jitraporn Vongsvivut; Olha Bazaka; Kateryna Bazaka; Rodney J Croft; Russell J Crawford; Elena P Ivanova Journal: Cancers (Basel) Date: 2019-01-31 Impact factor: 6.639