| Literature DB >> 2662972 |
K Watanabe1, S Kinoshita, H Nakagawa.
Abstract
Interleukin-1 beta, tumor necrosis factor-alpha and lipopolysaccharide stimulated normal rat kidney cell line (NRK-52E) to produce a chemotactic factor for rat neutrophils. This cytokine-induced neutrophil chemoattractant (CINC) was purified to obtain a single band with a M.W. of 63,000 on SDS-PAGE. The purified CINC induced detectable migration and strong chemotaxis of neutrophils at concentrations of 10(-9)M and 10(-7) - 10(-8)M, respectively. Checkerboard analysis indicated that CINC was a real chemotactic factor. Amino acid composition of CINC showed that CINC has a resemblance to human monocyte-derived neutrophil chemotactic factor (MDNCF) rather than human complement fragment, C5a.Entities:
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Year: 1989 PMID： 2662972 DOI： 10.1016/0006-291x(89)91355-7
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575