Xuefeng Wang1, Liyang Dong2, Yong Liang3, Hongchang Ni4, Jun Tang4, Chengcheng Xu2, Yuepeng Zhou2, Yuting Su2, Jun Wang4, Deyu Chen2, Chaoming Mao2. 1. Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University Zhenjiang 212001, P. R. China ; Institute of Oncology, The Affiliated Hospital of Jiangsu University Zhenjiang 212001, P. R. China ; Department of Rheumatology, The Affiliated People's Hospital, Jiangsu University Zhenjiang 212002, Jiangsu, P. R. China. 2. Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University Zhenjiang 212001, P. R. China ; Institute of Oncology, The Affiliated Hospital of Jiangsu University Zhenjiang 212001, P. R. China. 3. Clinical Laboratory, Huai'an Hospital Affiliated of Xuzhou Medical College Huaian, P. R. China. 4. Department of Rheumatology, The Affiliated People's Hospital, Jiangsu University Zhenjiang 212002, Jiangsu, P. R. China.
Abstract
OBJECTIVES: To compare the cytokine profile in RA patients and healthy control by using two methods-FlowCytomix assay and traditional ELISA. METHODS: Cytokine levels were evaluated by FlowCytomix assay and ELISA in serum and supernatants of peripheral blood mononuclear cells (PBMC) cultures with and without stimulation by phytohaemagglutinin (PHA). RESULTS: The levels of IL-6, IL-1β, and TNF-α were significantly higher in sera of RA patients than those of healthy controls. The levels of IL-22, IL-6, IL-1β, TNF-α, and IL-10 were higher in unstimulated PBMC culture supernatant of RA patients than those of healthy controls. PHA stimulation significantly increased the production of proinflammatory cytokines from PBMC with RA patients. Compared with detectable cytokine levels in sera, cytokine concentration in the supernatant of PBMCs was remarkably higher. FlowCytomix and ELISA showed significant correlation in detecting cytokines. However, the FlowCytomix assay detected more cytokines than ELISA. CONCLUSION: The supernatant of PBMCs provide a fine condition for the study of cytokine production because of the lack of interference factors in sera. The FlowCytomix assay is more sensitive than ELISA in detecting cytokines from RA patients. Multiple cytokine signatures using FlowCytomix assay may represent a more realistic approach in the future of personalized medicine in RA.
OBJECTIVES: To compare the cytokine profile in RApatients and healthy control by using two methods-FlowCytomix assay and traditional ELISA. METHODS: Cytokine levels were evaluated by FlowCytomix assay and ELISA in serum and supernatants of peripheral blood mononuclear cells (PBMC) cultures with and without stimulation by phytohaemagglutinin (PHA). RESULTS: The levels of IL-6, IL-1β, and TNF-α were significantly higher in sera of RApatients than those of healthy controls. The levels of IL-22, IL-6, IL-1β, TNF-α, and IL-10 were higher in unstimulated PBMC culture supernatant of RApatients than those of healthy controls. PHA stimulation significantly increased the production of proinflammatory cytokines from PBMC with RApatients. Compared with detectable cytokine levels in sera, cytokine concentration in the supernatant of PBMCs was remarkably higher. FlowCytomix and ELISA showed significant correlation in detecting cytokines. However, the FlowCytomix assay detected more cytokines than ELISA. CONCLUSION: The supernatant of PBMCs provide a fine condition for the study of cytokine production because of the lack of interference factors in sera. The FlowCytomix assay is more sensitive than ELISA in detecting cytokines from RApatients. Multiple cytokine signatures using FlowCytomix assay may represent a more realistic approach in the future of personalized medicine in RA.
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