Literature DB >> 2662814

Cyanogen bromide cleavage at methionine residues of polypeptides containing disulfide bonds.

S Villa1, G De Fazio, U Canosi.   

Abstract

A method for cleaving polypeptides at their methionine residues without affecting intramolecular disulfide bonds is described. This method may be applied for cleaving recombinant heterologous hybrid polypeptides with release of the interesting peptide. The method may also be applied to assign the correct positions of disulfide bonds in protein molecules.

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Year:  1989        PMID： 2662814     DOI： 10.1016/0003-2697(89)90032-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Reactive oxygen species and nitric oxide mediate plasticity of neuronal calcium signaling.

Authors:  O Yermolaieva; N Brot; H Weissbach; S H Heinemann; T Hoshi
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-04       Impact factor: 11.205

2.  Localization of human intestinal defensin 5 in Paneth cell granules.

Authors:  E M Porter; L Liu; A Oren; P A Anton; T Ganz
Journal:  Infect Immun       Date:  1997-06       Impact factor: 3.441

3.  An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I.

Authors:  G Forsberg; B Baastrup; H Rondahl; E Holmgren; G Pohl; M Hartmanis; M Lake
Journal:  J Protein Chem       Date:  1992-04

4.  Intramolecular inhibition of human defensin HNP-1 by its propiece.

Authors:  E V Valore; E Martin; S S Harwig; T Ganz
Journal:  J Clin Invest       Date:  1996-04-01       Impact factor: 14.808

5.  The complete primary structure of ribosomal protein L1 from Thermus thermophilus.

Authors:  R Amons; T A Muranova; A I Rykunova; I A Eliseikina; S E Sedelnikova
Journal:  J Protein Chem       Date:  1993-12

6.  Predominant cleavage of proteins N-terminal to serines and threonines using scandium(III) triflate.

Authors:  Christian J Koehler; Bernd Thiede
Journal:  J Biol Inorg Chem       Date:  2019-10-30       Impact factor: 3.358
  6 in total

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