Silvia Ferronato1, Matteo Gelati1, Alberto Scuro2, Silvia Olivato1, Giovanni Malerba1, Maria Grazia Romanelli3, Macarena Gomez-Lira1, Carlo Setacci4. 1. Department of Neurological, Biomedical and Movement Sciences, Section of Biology and Genetics, University of Verona, Strada Le Grazie, 8, 37134, Verona, Italy. 2. Department of Surgery, University of Verona, Verona, Italy. 3. Department of Neurological, Biomedical and Movement Sciences, Section of Biology and Genetics, University of Verona, Strada Le Grazie, 8, 37134, Verona, Italy. mariagrazia.romanelli@univr.it. 4. Division of Vascular Surgery, University of Siena, Siena, Italy.
Abstract
BACKGROUND AND OBJECTIVES: A variant located at the end of HDAC9 gene within clusters of DNAse I sensitivity zones and histone modification hotspots has been associated with large vessel stroke and could be linked to plaque instability. The aim of the study is to define if an altered expression of HDAC9, TWIST1 and FERD3L genes could be involved in plaque vulnerability. METHODS: Histological classification and gene expression analysis were performed in 6 stable and 16 unstable plaques obtained from asymptomatic patients undergoing endarterectomy. Gene expression was analysed by real-time PCR. RESULTS AND CONCLUSIONS: TWIST1 gene expression resulted higher in stable plaques (P < 0.02). HDAC9 gene expression followed a similar trend (P = 0.11). These results highlighting the significant correlation between TWIST and HDAC9 gene expression suggest that both genes may contribute to plaque stability in a coordinated way.
BACKGROUND AND OBJECTIVES: A variant located at the end of HDAC9 gene within clusters of DNAse I sensitivity zones and histone modification hotspots has been associated with large vessel stroke and could be linked to plaque instability. The aim of the study is to define if an altered expression of HDAC9, TWIST1 and FERD3L genes could be involved in plaque vulnerability. METHODS: Histological classification and gene expression analysis were performed in 6 stable and 16 unstable plaques obtained from asymptomatic patients undergoing endarterectomy. Gene expression was analysed by real-time PCR. RESULTS AND CONCLUSIONS:TWIST1 gene expression resulted higher in stable plaques (P < 0.02). HDAC9 gene expression followed a similar trend (P = 0.11). These results highlighting the significant correlation between TWIST and HDAC9 gene expression suggest that both genes may contribute to plaque stability in a coordinated way.
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