| Literature DB >> 26619873 |
Jaime A Teixeira da Silva1, Budi Winarto2.
Abstract
The protocorm-like body (PLB) is the de facto somatic embryo in orchids. Here we describe detailed protocols for two orchid genera (hybrid Cymbidium Twilight Moon 'Day Light' and Dendrobium 'Jayakarta', D. 'Gradita 31', and D. 'Zahra FR 62') for generating PLBs. These protocols will most likely have to be tweaked for different cultivars as the response of orchids in vitro tends to be dependent on genotype. In addition to primary somatic embryogenesis, secondary (or repetitive) somatic embryogenesis is also described for both genera. The use of thin cell layers as a sensitive tissue assay is outlined for hybrid Cymbidium while the protocol outlined is suitable for bioreactor culture of D. 'Zahra FR 62'.Keywords: Culture system; Dendrobium; Hybrid Cymbidium; In vitro propagation; Plant growth regulator s; Protocorm-like body; Somatic embryo; Thin cell layer; Tissue culture
Mesh：
Year: 2016 PMID： 26619873 DOI： 10.1007/978-1-4939-3061-6_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745