Jinyou Han1, Hong He2. 1. Department of Orthodontics, School & Hospital of Stomatology, Wuhan University Hubei Province, Wuhan 430079, China ; Liaocheng People's Hospital Shandong Province, China. 2. Department of Orthodontics, School & Hospital of Stomatology, Wuhan University Hubei Province, Wuhan 430079, China.
Abstract
OBJECTIVE: To investigate the expression and function of osteogenic genes osterix (OSX) and runt-related transcription factor 2 (RUNX2) in the rat periodontal tissues under orthodontic force for the remodeling of the periodontal tissues. METHODS: 24 Wistar rats were randomly divided into 4 groups of orthodontic tooth movements for 1, 3, and 7 days (experimental groups) and control group (without orthodontic force). The expression of RUNX2 and OSX in the periodontal tissues was analyzed using real time PCR for mRNA and Western blot analysis for protein. The data were also analyzed for involvement of the two genes in signal pathways using bioinformatics tools. RESULTS: The mRNA levels of RUNX2 and OSX increased in the periodontal tissues after subjected to the orthodontic force for 1 to 7 days, with the highest level occurring at day 7. The relative expression levels of RUNX2 and OSX mRNA were 1.85 ± 0.12, 304 ± 0.06 and 4.16 ± 0.068, and 1.52 ± 0.09, 1.83 ± 0.03 and 2.56 ± 0.06 at day 1, 3 and 7, respectively. The results of Western blot analysis were consistent with the mRNA results. CONCLUSION: In orthodontic tooth movement, the expression of RUNX2 and OSX was upregulated as a result of external stimulation, suggesting that the two genes is involved in periodontal tissue remodeling and plays an important role in periodontal tissue remodeling.
OBJECTIVE: To investigate the expression and function of osteogenic genes osterix (OSX) and runt-related transcription factor 2 (RUNX2) in the rat periodontal tissues under orthodontic force for the remodeling of the periodontal tissues. METHODS: 24 Wistar rats were randomly divided into 4 groups of orthodontic tooth movements for 1, 3, and 7 days (experimental groups) and control group (without orthodontic force). The expression of RUNX2 and OSX in the periodontal tissues was analyzed using real time PCR for mRNA and Western blot analysis for protein. The data were also analyzed for involvement of the two genes in signal pathways using bioinformatics tools. RESULTS: The mRNA levels of RUNX2 and OSX increased in the periodontal tissues after subjected to the orthodontic force for 1 to 7 days, with the highest level occurring at day 7. The relative expression levels of RUNX2 and OSX mRNA were 1.85 ± 0.12, 304 ± 0.06 and 4.16 ± 0.068, and 1.52 ± 0.09, 1.83 ± 0.03 and 2.56 ± 0.06 at day 1, 3 and 7, respectively. The results of Western blot analysis were consistent with the mRNA results. CONCLUSION: In orthodontic tooth movement, the expression of RUNX2 and OSX was upregulated as a result of external stimulation, suggesting that the two genes is involved in periodontal tissue remodeling and plays an important role in periodontal tissue remodeling.
Authors: K Thirunavukkarasu; D L Halladay; R R Miles; X Yang; R J Galvin; S Chandrasekhar; T J Martin; J E Onyia Journal: J Biol Chem Date: 2000-08-18 Impact factor: 5.157