Conghui Wang1, Qi Jiang1, Min Wang1, Dong Li1. 1. Department of Anesthesiology, The First Hospital of Xiangyang City Xiangyang 441000, Hubei Province, China.
Abstract
PURPOSE: We aimed to investigate whether miR-19a is associated with neuropathic pain and elucidate the underlying regulatory mechanism. METHODS: We established a neuropathic pain model of bilateral chronic constriction injury (bCCI). Then bCCI rats were injected with mo-miR-19a, siR-SOCS1 or blank expression vector through a microinjection syringe via an intrathecal catheter on 3 day before surgery and after surgery. Behavioral tests, such as mechanical allodynia, thermal hyperalgesia and acetone induced cold allodynia, were performed to evaluate the pain threshold. Besides, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the expression of miR-19a and western blotting was carried out to measure the expression of SOCS1. RESULTS: miR-19a expression levels were markedly increased in neuropathic pain models. Moreover, miR-19a significantly attenuated mechanical allodynia and thermal hyperalgesia, and similar results were obtained after knockdown of SOCS1 expression. However, miR-19a markedly increased the times that the rats appeared a sign of cold allodynia, and knockdown of SOCS1 expression had similar effects. Besides, the results of bioinformatics analysis and western blotting analysis were all confirmed that SOCS1 was a direct target of miR-19a in neuropathic pain models. CONCLUSIONS: Our finding indicate that SOCS1 is a direct target of miR-19a in neuropathic pain rats and miR-19a may play a critical role in regulating of neuropathic pain via targeting SOCS1.
PURPOSE: We aimed to investigate whether miR-19a is associated with neuropathic pain and elucidate the underlying regulatory mechanism. METHODS: We established a neuropathic pain model of bilateral chronic constriction injury (bCCI). Then bCCI rats were injected with mo-miR-19a, siR-SOCS1 or blank expression vector through a microinjection syringe via an intrathecal catheter on 3 day before surgery and after surgery. Behavioral tests, such as mechanical allodynia, thermal hyperalgesia and acetone induced cold allodynia, were performed to evaluate the pain threshold. Besides, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the expression of miR-19a and western blotting was carried out to measure the expression of SOCS1. RESULTS:miR-19a expression levels were markedly increased in neuropathic pain models. Moreover, miR-19a significantly attenuated mechanical allodynia and thermal hyperalgesia, and similar results were obtained after knockdown of SOCS1 expression. However, miR-19a markedly increased the times that the rats appeared a sign of cold allodynia, and knockdown of SOCS1 expression had similar effects. Besides, the results of bioinformatics analysis and western blotting analysis were all confirmed that SOCS1 was a direct target of miR-19a in neuropathic pain models. CONCLUSIONS: Our finding indicate that SOCS1 is a direct target of miR-19a in neuropathic painrats and miR-19a may play a critical role in regulating of neuropathic pain via targeting SOCS1.
Authors: Annemarie Ledeboer; Michael Gamanos; Wenmin Lai; David Martin; Steven F Maier; Linda R Watkins; Ning Quan Journal: Eur J Neurosci Date: 2005-10 Impact factor: 3.386
Authors: David von Schack; Michael J Agostino; B Stuart Murray; Yizheng Li; Padmalatha S Reddy; Jin Chen; Sung E Choe; Brian W Strassle; Christine Li; Brian Bates; Lynn Zhang; Huijuan Hu; Smita Kotnis; Brendan Bingham; Wei Liu; Garth T Whiteside; Tarek A Samad; Jeffrey D Kennedy; Seena K Ajit Journal: PLoS One Date: 2011-03-14 Impact factor: 3.240
Authors: Sarah D Linnstaedt; Cathleen A Rueckeis; Kyle D Riker; Yue Pan; Alan Wu; Shan Yu; Britannia Wanstrath; Michael Gonzalez; Evan Harmon; Paul Green; Chieh V Chen; Tony King; Christopher Lewandowski; Phyllis L Hendry; Claire Pearson; Michael C Kurz; Elizabeth Datner; Marc-Anthony Velilla; Robert Domeier; Israel Liberzon; Jeffrey S Mogil; Jon Levine; Samuel A McLean Journal: Pain Date: 2020-01 Impact factor: 6.961
Authors: James M Elliott; Cathleen A Rueckeis; Yue Pan; Todd B Parrish; David M Walton; Sarah D Linnstaedt Journal: Sci Rep Date: 2021-02-04 Impact factor: 4.379
Authors: Liang Ye; Leslie R Morse; Scott P Falci; Julie K Olson; Mayank Shrivastava; Nguyen Nguyen; Clas Linnman; Karen L Troy; Ricardo A Battaglino Journal: Neurotrauma Rep Date: 2021-09-14