| Literature DB >> 26617766 |
Tianjie Pu1, Peng Guo2, Yan Qiu1, Shinan Chen2, Libo Yang1, Linyong Sun3, Feng Ye2, Hong Bu1.
Abstract
Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are the most common methods that are used to quantify HER-2 gene and protein levels, respectively, in human breast cancer. However, due to bad sample quality, some samples are unable to be subjected to a FISH assay. We evaluated 71 formalin-fixed paraffin-embedded (FFPE) breast carcinoma specimens by quantitative real-time polymerase chain reaction (qPCR), IHC, and FISH. We also performed qPCR and FISH assays on delayed formalin-fixed (DDF) samples. The qPCR results were in complete concordance with the results of IHC and FISH. In regards to the DDF samples, the HER-2 fluorescent signal seemed decayed compared with that of the DDF samples after 1 h. However, the qPCR method still works well up to 12 hours. Our results indicated that qPCR was obviously superior to FISH in cases that were not fixed in a reasonable amount of time. However, qPCR can be an alternative method by which to perform HER2 amplification assays in breast cancer.Entities:
Keywords: FISH; HER-2 copy number variation; IHC; breast neoplasm; delayed formalin-fixed; quantitative real-time polymerase chain reaction
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Year: 2015 PMID： 26617766 PMCID： PMC4637581
Source DB: PubMed Journal: Int J Clin Exp Pathol ISSN： 1936-2625