Literature DB >> 2661585

Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets.

S Linde1, J H Nielsen, B Hansen, B S Welinder.   

Abstract

Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.

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Year:  1989        PMID： 2661585     DOI： 10.1016/s0021-9673(00)91351-7

Source DB:  PubMed          Journal:  J Chromatogr


  7 in total

1.  Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion.

Authors:  C M Buchanan; A R Phillips; G J Cooper
Journal:  Biochem J       Date:  2001-12-01       Impact factor: 3.857

2.  Knock-in of diphteria toxin A chain gene at Ins2 locus: effects on islet development and localization of Ins2 expression in the brain.

Authors:  Luciane Lamotte; Malene Jackerott; Danielle Bucchini; Jacques Jami; Rajiv L Joshi; Louise Deltour
Journal:  Transgenic Res       Date:  2004-10       Impact factor: 2.788

3.  Adaptation to supraphysiologic levels of insulin gene expression in transgenic mice: evidence for the importance of posttranscriptional regulation.

Authors:  B Schnetzler; G Murakawa; D Abalos; P Halban; R Selden
Journal:  J Clin Invest       Date:  1993-07       Impact factor: 14.808

4.  Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II.

Authors:  S V Sizonenko; P A Halban
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857

5.  Differential expression of the two nonallelic proinsulin genes in the developing mouse embryo.

Authors:  L Deltour; P Leduque; N Blume; O Madsen; P Dubois; J Jami; D Bucchini
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-15       Impact factor: 11.205

6.  A specific and sensitive radioimmunoassay for rat C-peptide.

Authors:  J O Akpan; L G Weide; R L Gingerich
Journal:  Int J Pancreatol       Date:  1993-04

7.  Hyper-Variability in Circulating Insulin, High Fat Feeding Outcomes, and Effects of Reducing Ins2 Dosage in Male Ins1-Null Mice in a Specific Pathogen-Free Facility.

Authors:  Nicole M Templeman; Arya E Mehran; James D Johnson
Journal:  PLoS One       Date:  2016-04-07       Impact factor: 3.240
  7 in total

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