Po-Jen Yang1,2,3,4, Wei-Shiung Yang1,4,5, Hsiao-Ching Nien1,6, Chiung-Nien Chen3,4, Po-Huang Lee3, Linda Chia-Hui Yu7, Ming-Tsan Lin8. 1. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan. 2. Department of Surgery, National Taiwan University Hospital Jin-Shan Branch, New Taipei City, Taiwan. 3. Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan. 4. Center for Obesity, Life Style and Metabolic Surgery, National Taiwan University Hospital, Taipei, Taiwan. 5. Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan. 6. Department of Family Medicine, National Taiwan University Hospital, Taipei, Taiwan. 7. Graduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, Taiwan. lchyu@ntu.edu.tw. 8. Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan. linmt@ntu.edu.tw.
Abstract
BACKGROUND: Gastrointestinal bypass changes the gut microbiota and decreases systemic endotoxemia in obese subjects. Epithelial barrier integrity is crucial for confining enteric bacteria in the lumen and preventing gut-derived endotoxemia. The effect of bypass surgery on intestinal barrier functions remains poorly understood. This study aimed to evaluate the changes in intestinal permeability and gut barrier between rats receiving Roux-en-Y duodenojejunal bypass (DJB) or sham operation (SO). METHODS: Eighteen Sprague-Dawley rats were assigned to DJB or SO groups. Tissues of the alimentary, biliopancreatic, and common limbs in the small intestine, and the colon, were collected 2 weeks after operation. Mucosa-associated bacteria were quantified by colony forming units. Intestinal permeability was determined by mucosal-to-serosal dextran flux measured in Ussing chambers. Expression of occludin and proliferating cell nuclear antigen (PCNA) in the intestinal mucosa was examined by western blots. RESULTS: Enteric bacterial numbers were increased in the alimentary and common limbs after DJB. Reduced dextran permeability was found in the alimentary limb, common limb, and colon after DJB. Moreover, increased villus height and crypt depth were found to be associated with higher mucosal levels of occludin and PCNA levels in the alimentary and common limbs after DJB. CONCLUSIONS: DJB in rats altered gut microbiota and reduced intestinal permeability due to increased epithelial proliferation and tight junctional protein expression. Our results show that bypass surgery led to fortification of the intestinal barrier functions, which may provide an explanation for the decreased risk of systemic endotoxemia in postoperative patients.
BACKGROUND: Gastrointestinal bypass changes the gut microbiota and decreases systemic endotoxemia in obese subjects. Epithelial barrier integrity is crucial for confining enteric bacteria in the lumen and preventing gut-derived endotoxemia. The effect of bypass surgery on intestinal barrier functions remains poorly understood. This study aimed to evaluate the changes in intestinal permeability and gut barrier between rats receiving Roux-en-Y duodenojejunal bypass (DJB) or sham operation (SO). METHODS: Eighteen Sprague-Dawley rats were assigned to DJB or SO groups. Tissues of the alimentary, biliopancreatic, and common limbs in the small intestine, and the colon, were collected 2 weeks after operation. Mucosa-associated bacteria were quantified by colony forming units. Intestinal permeability was determined by mucosal-to-serosal dextran flux measured in Ussing chambers. Expression of occludin and proliferating cell nuclear antigen (PCNA) in the intestinal mucosa was examined by western blots. RESULTS: Enteric bacterial numbers were increased in the alimentary and common limbs after DJB. Reduced dextran permeability was found in the alimentary limb, common limb, and colon after DJB. Moreover, increased villus height and crypt depth were found to be associated with higher mucosal levels of occludin and PCNA levels in the alimentary and common limbs after DJB. CONCLUSIONS: DJB in rats altered gut microbiota and reduced intestinal permeability due to increased epithelial proliferation and tight junctional protein expression. Our results show that bypass surgery led to fortification of the intestinal barrier functions, which may provide an explanation for the decreased risk of systemic endotoxemia in postoperative patients.
Authors: Scott V Monte; Joseph A Caruana; Husam Ghanim; Chang Ling Sia; Kelly Korzeniewski; Jerome J Schentag; Paresh Dandona Journal: Surgery Date: 2011-11-16 Impact factor: 3.982
Authors: Almino C Ramos; Manoel P Galvão Neto; Yglésio Moyses de Souza; Manoela Galvão; Abel H Murakami; Andrey C Silva; Edwin G Canseco; Raúl Santamaría; Trino A Zambrano Journal: Obes Surg Date: 2008-11-06 Impact factor: 4.129
Authors: Torsten P M Scheithauer; Mark Davids; Maaike Winkelmeijer; Xanthe Verdoes; Ömrüm Aydin; Maurits de Brauw; Arnold van de Laar; Abraham S Meijnikman; Victor E A Gerdes; Daniël van Raalte; Hilde Herrema; Max Nieuwdorp Journal: Gut Microbes Date: 2022 Jan-Dec