Literature DB >> 2661316

Cloning and expression of a Clostridium acetobutylicum alpha-amylase gene in Escherichia coli.

P Verhasselt1, F Poncelet, K Vits, A Van Gool, J Vanderleyden.   

Abstract

A gene library of Clostridium acetobutylicum ATCC824 was constructed in the plasmid vector pEcoR251. The library was tested for the presence of starch hydrolyzing clones. One clone in which the recombinant plasmid, pVP101, conferred alpha-amylase activity to the Escherichia coli host cell, was detected. The gene is carried on a 3.45-kbp BglII restriction fragment. A detailed physical map of pVP101 is presented.

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Year:  1989        PMID： 2661316     DOI： 10.1111/j.1574-6968.1989.tb03097.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

1.  A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils.

Authors:  Dennis Claessen; Rick Rink; Wouter de Jong; Jeroen Siebring; Peter de Vreugd; F G Hidde Boersma; Lubbert Dijkhuizen; Han A B Wosten
Journal:  Genes Dev       Date:  2003-06-27       Impact factor: 11.361

2.  Targeted disruption of the kstD gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1.

Authors:  R van Der Geize; G I Hessels; R van Gerwen; J W Vrijbloed; P van Der Meijden; L Dijkhuizen
Journal:  Appl Environ Microbiol       Date:  2000-05       Impact factor: 4.792

3.  Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum.

Authors:  U Gerischer; P Dürre
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

4.  Characterization of a novel fructosyltransferase from Lactobacillus reuteri that synthesizes high-molecular-weight inulin and inulin oligosaccharides.

Authors:  S A F T van Hijum; G H van Geel-Schutten; H Rahaoui; M J E C van der Maarel; L Dijkhuizen
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

5.  The probiotic Lactobacillus johnsonii NCC 533 produces high-molecular-mass inulin from sucrose by using an inulosucrase enzyme.

Authors:  Munir A Anwar; Slavko Kralj; Marc J E C van der Maarel; Lubbert Dijkhuizen
Journal:  Appl Environ Microbiol       Date:  2008-04-11       Impact factor: 4.792

6.  Degradation of the thiocarbamate herbicide EPTC (S-ethyl dipropylcarbamothioate) and biosafening by Rhodococcus sp. strain NI86/21 involve an inducible cytochrome P-450 system and aldehyde dehydrogenase.

Authors:  I Nagy; G Schoofs; F Compernolle; P Proost; J Vanderleyden; R de Mot
Journal:  J Bacteriol       Date:  1995-02       Impact factor: 3.490

7.  Effect of acetate on molecular and physiological aspects of Clostridium beijerinckii NCIMB 8052 solvent production and strain degeneration.

Authors:  C K Chen; H P Blaschek
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792
  7 in total

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