Liqing Sun1, Zhihong Xu2, Wei Huang3,4, Shanshan Wu2, Xinheng Lin1, Fengyu Zhu1, Nengrong Liu2, Meizhen Huang5, Rong Chen2, Haishan Zeng6. 1. Affiliated Fuzhou First Hospital of Fujian Medical University, Fuzhou, 350009, China. 2. Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. 3. Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, 350007, China. huang84wei@163.com. 4. Fujian Metrology Institute, Fuzhou, 350003, China. huang84wei@163.com. 5. Institute of Optical Engineering, Department of Physics, Shanghai Jiao Tong University, Shanghai, 200240, China. 6. Imaging Unit - Integrative Oncology Department, British Columbia Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada.
Abstract
PURPOSE: Current practice for diagnosing nasopharyngeal carcinoma (NPC) is based on invasive tissue biopsy. This study aims to explore the feasibility of using Raman spectroscopy to differentiate cancerous and non-cancerous nasopharyngeal tissue smears, expecting to realize minimal invasive diagnosis using smears from in vivo mucosa tissue by Raman spectroscopy. METHODS: Biopsy tissue smears were acquired from 74 patients with pathologically diagnosed nasopharyngeal diseases and measured using confocal Raman spectroscopy. RESULTS: Both fingerprint region and high wavenumber Raman spectra were acquired with distinguish features. Multivariate statistical analysis was used to differentiate cancerous and non-cancerous groups, achieving a diagnostic sensitivity of 87.2 and specificity of 85.7 % for differentiating NPC from nasopharyngeal non-cancerous smears. CONCLUSIONS: This work indicates that the method has a unique advantage in microanalysis for tissue smears which may provide a promising minimal invasive (or noninvasive) diagnosing tool for cancer diagnosis.
PURPOSE: Current practice for diagnosing nasopharyngeal carcinoma (NPC) is based on invasive tissue biopsy. This study aims to explore the feasibility of using Raman spectroscopy to differentiate cancerous and non-cancerous nasopharyngeal tissue smears, expecting to realize minimal invasive diagnosis using smears from in vivo mucosa tissue by Raman spectroscopy. METHODS: Biopsy tissue smears were acquired from 74 patients with pathologically diagnosed nasopharyngeal diseases and measured using confocal Raman spectroscopy. RESULTS: Both fingerprint region and high wavenumber Raman spectra were acquired with distinguish features. Multivariate statistical analysis was used to differentiate cancerous and non-cancerous groups, achieving a diagnostic sensitivity of 87.2 and specificity of 85.7 % for differentiating NPC from nasopharyngeal non-cancerous smears. CONCLUSIONS: This work indicates that the method has a unique advantage in microanalysis for tissue smears which may provide a promising minimal invasive (or noninvasive) diagnosing tool for cancer diagnosis.
Authors: Zhiwei Huang; Annette McWilliams; Harvey Lui; David I McLean; Stephen Lam; Haishan Zeng Journal: Int J Cancer Date: 2003-12-20 Impact factor: 7.396