A Asdadi1, A Hamdouch2, A Oukacha3, R Moutaj4, S Gharby5, H Harhar5, M El Hadek6, B Chebli7, L M Idrissi Hassani2. 1. Équipe Planta Sud, laboratoire de biotechnologies végétales, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco. Electronic address: asdadiali@gmail.com. 2. Équipe Planta Sud, laboratoire de biotechnologies végétales, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco. 3. Laboratoire de biotechnologies végétales, lutte biologique et phytochimie, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco. 4. Laboratoire de parasitologie et de mycologie, hôpital militaire Avicenne, CHU Med VI, 40000 Marrakech, Morocco. 5. Laboratoire de chimie des plantes et de synthèse organique et bioorganique, faculté des sciences, université Mohammed V-Agdal, BP 1014, Rabat, Morocco. 6. Laboratoire de génie des procédés, faculté des sciences d'Agadir, université Ibn Zohr, BP 28/S, Agadir, Morocco. 7. Laboratoire de mécanique de procédé de l'énergie et de l'environnement, école nationale des sciences appliquées, université Ibn Zohr, BP 1136, Agadir, Morocco.
Abstract
OBJECTIVE: To study the composition, the antioxidant activity and the in vitro antifungal action anti-Candida species of essential oils extracted from seeds of Vite xagnus-castus L. MATERIALS AND METHOD: The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was analyzed using the DPPH free radical-scavenging method. Susceptibility tests for Candida albicans (12), C. dubliniensis (1), C. glabrata (3), C. krusei (3), C. parapsilosis (6), C. lusitaniae (1), C. famata (1) and C. tropicalis (3) were expressed as inhibition zone by the disc-diffusion method and as minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method, compared to amphotricin B and fluconazol as standard drugs. RESULTS: Major components were: 1,8-cineole (19.61%), sabinene (14.57%), α-pinene (9.76%), β-farnesene (6.04%), β-caryophyllene oxide (5.83%) and β-caryophyllene (5.02%). A low antioxidant activity was found (IC50=1.072mg/ml), but it can be exploited. V. agnus-castus seeds essential oils disosed a 35-58mm zone of inhibition (mean: 49mm) against all 30 isolates tested. In broth macrodilution method, all the tested Candida species were susceptible to the essential oils and this activity was concentration-dependent. MIC values varied from 0.13 to 2.13mg/ml V. agnus-castus seeds essential oils. CONCLUSION: Results of this study indicated that the oils of plant origin could be used as potential anti-Candida species causative agents of nosocomial infections. These oils exhibited a noticeable antifungal activity against the selected fungi. The natural antifungal substances are inexpensive and have fewer side effects, they may represent alternative therapies for candidiasis.
OBJECTIVE: To study the composition, the antioxidant activity and the in vitro antifungal action anti-Candida species of essential oils extracted from seeds of Vite xagnus-castus L. MATERIALS AND METHOD: The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was analyzed using the DPPH free radical-scavenging method. Susceptibility tests for Candida albicans (12), C. dubliniensis (1), C. glabrata (3), C. krusei (3), C. parapsilosis (6), C. lusitaniae (1), C. famata (1) and C. tropicalis (3) were expressed as inhibition zone by the disc-diffusion method and as minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method, compared to amphotricin B and fluconazol as standard drugs. RESULTS: Major components were: 1,8-cineole (19.61%), sabinene (14.57%), α-pinene (9.76%), β-farnesene (6.04%), β-caryophyllene oxide (5.83%) and β-caryophyllene (5.02%). A low antioxidant activity was found (IC50=1.072mg/ml), but it can be exploited. V. agnus-castus seeds essential oils disosed a 35-58mm zone of inhibition (mean: 49mm) against all 30 isolates tested. In broth macrodilution method, all the tested Candida species were susceptible to the essential oils and this activity was concentration-dependent. MIC values varied from 0.13 to 2.13mg/ml V. agnus-castus seeds essential oils. CONCLUSION: Results of this study indicated that the oils of plant origin could be used as potential anti-Candida species causative agents of nosocomial infections. These oils exhibited a noticeable antifungal activity against the selected fungi. The natural antifungal substances are inexpensive and have fewer side effects, they may represent alternative therapies for candidiasis.
Authors: Magaiver Andrade-Silva; Luana Barbosa Correa; André Luis Peixoto Candéa; Simone C Cavalher-Machado; Helene Santos Barbosa; Elaine Cruz Rosas; Maria G Henriques Journal: Inflamm Res Date: 2016-07-05 Impact factor: 4.575