Literature DB >> 26606726

Correction: Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

Graziela Murta Barbosa, Andrea Vieira Colombo, Paulo Henrique Rodrigues, Maria Regina Lorenzetti Simionato.   

Abstract

Entities:  

Year:  2015        PMID: 26606726      PMCID: PMC4659679          DOI: 10.1371/journal.pone.0143903

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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The image for Fig 3 is missing Fig 3B and 3C. Please see the corrected Fig 3 here.
Fig 3

Effect of co-culture of Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens on biofilm formation in a two-compartment co-culture system.

Panel A: P. gingivalis ATCC33277 and co-culture partners P. intermedia strains 17 and ATCC25611, and P. nigrescens ATCC33563. Panel B: P. gingivalis W83 and co-culture partners P. intermedia strains 17 and ATCC25611, and P. nigrescens ATCC33563. Panel C: P. intermedia strains 17 and ATCC25611 and co-culture partner P. nigrescens ATCC33563 Values represent average of 3 experiments (Kruskal-Wallis test followed by nonparametric post-tests, *p < 0.01). a denotes strain in the lower compartment (biomass measured) and b denotes strain in the upper compartment.

Effect of co-culture of Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens on biofilm formation in a two-compartment co-culture system.

Panel A: P. gingivalis ATCC33277 and co-culture partners P. intermedia strains 17 and ATCC25611, and P. nigrescens ATCC33563. Panel B: P. gingivalis W83 and co-culture partners P. intermedia strains 17 and ATCC25611, and P. nigrescens ATCC33563. Panel C: P. intermedia strains 17 and ATCC25611 and co-culture partner P. nigrescens ATCC33563 Values represent average of 3 experiments (Kruskal-Wallis test followed by nonparametric post-tests, *p < 0.01). a denotes strain in the lower compartment (biomass measured) and b denotes strain in the upper compartment. Table 1 has been corrected for improved readability. Please see the corrected Table 1 here.
Table 1

Primer sequences used for quantitative real-time PCR (q-PCR).

Target genesPrimers (5’– 3’)Product size (bp)Locus tag (NIH)
P. gingivalis waaA 1 F: TGGTTTCATGCAGCTTCTTT146PGN_RS02590
R: TCGGCACCTTCGTAATTCTT
P. intermedia waaA 1 F:GACCCGAACGCAAAATACAT130PIN17_RS06380
R:AGGGCGAAAAGAACGTTAGG
P. nigrescens waaA 2 F: GCTGCTGGACACTCCAAGGCTTT120ZP 08672979
R: GCTATGATGAGTTTCCACTCGCTGTG
P. gingivalis thiC 2 F: ACGAYGCCAAYGATGCTGCPGN_RS00750
P. intermedia thiC 2 R: ATCTTGTGCATBGGYACGTGTCC124PIN17_RS04875
P. nigrescens thiC 2 ZP 08673195

Source:

1 Hyvarinen et al., 2009;

2 This study

Source: 1 Hyvarinen et al., 2009; 2 This study
  1 in total

1.  Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

Authors:  Graziela Murta Barbosa; Andrea Vieira Colombo; Paulo Henrique Rodrigues; Maria Regina Lorenzetti Simionato
Journal:  PLoS One       Date:  2015-09-25       Impact factor: 3.240

  1 in total

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