| Literature DB >> 26606726 |
Graziela Murta Barbosa, Andrea Vieira Colombo, Paulo Henrique Rodrigues, Maria Regina Lorenzetti Simionato.
Abstract
Entities:
Year: 2015 PMID: 26606726 PMCID: PMC4659679 DOI: 10.1371/journal.pone.0143903
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 3Effect of co-culture of Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens on biofilm formation in a two-compartment co-culture system.
Panel A: P. gingivalis ATCC33277 and co-culture partners P. intermedia strains 17 and ATCC25611, and P. nigrescens ATCC33563. Panel B: P. gingivalis W83 and co-culture partners P. intermedia strains 17 and ATCC25611, and P. nigrescens ATCC33563. Panel C: P. intermedia strains 17 and ATCC25611 and co-culture partner P. nigrescens ATCC33563 Values represent average of 3 experiments (Kruskal-Wallis test followed by nonparametric post-tests, *p < 0.01). a denotes strain in the lower compartment (biomass measured) and b denotes strain in the upper compartment.
Primer sequences used for quantitative real-time PCR (q-PCR).
| Target genes | Primers (5’– 3’) | Product size (bp) | Locus tag (NIH) |
|---|---|---|---|
|
| F: TGGTTTCATGCAGCTTCTTT | 146 | PGN_RS02590 |
| R: TCGGCACCTTCGTAATTCTT | |||
|
| F:GACCCGAACGCAAAATACAT | 130 | PIN17_RS06380 |
| R:AGGGCGAAAAGAACGTTAGG | |||
|
| F: GCTGCTGGACACTCCAAGGCTTT | 120 | ZP 08672979 |
| R: GCTATGATGAGTTTCCACTCGCTGTG | |||
|
| F: ACGAYGCCAAYGATGCTGC | PGN_RS00750 | |
|
| R: ATCTTGTGCATBGGYACGTGTCC | 124 | PIN17_RS04875 |
|
| ZP 08673195 |
Source:
1 Hyvarinen et al., 2009;
2 This study