Literature DB >> 26604410

Extraction, partial purification and determination of some biochemical properties of β-glucosidase from Tea Leaves (Camellia sinensis L.).

Aysun Şener1.   

Abstract

This research was carried out to determine biochemical properties of β-glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21) isolated from Turkish tea leaves. Two protein peaks containing β-glucosidase activity were recovered and characterized, which were denoted as isoenzyme A and isoenzyme B. Their pH optimum, thermal resistances, affinity towards p-nitrophenyl-β-D-glucopyranoside differed markedly. They both displayed maximal activity at pH 5.0. The effects of the inhibitors tested varied in a dose dependent manner.

Entities:  

Keywords:  Kinetics; Purification; Tea leaves; Thermal inactivation; Thermal stability; β-glucosidase

Year:  2015        PMID： 26604410      PMCID： PMC4648895          DOI： 10.1007/s13197-015-1915-z

Source DB:  PubMed          Journal:  J Food Sci Technol        ISSN： 0022-1155            Impact factor:   2.701


  11 in total

1.  Influence of green tea on enzymes of carbohydrate metabolism, antioxidant defense, and plasma membrane in rat tissues.

Authors:  Sara Anees Khan; Shubha Priyamvada; Natarajan A Arivarasu; Sheeba Khan; Ahad Noor Khan Yusufi
Journal:  Nutrition       Date:  2007-09       Impact factor: 4.008

2.  Purification and partial characterization of beta-glucosidase from fresh leaves of tea plants (Camellia sinensis (L.) O. Kuntze).

Authors:  Ye-Yun Li; Chang-Jun Jiang; Xiao-Chun Wan; Zheng-Zhu Zhang; Da-Xiang Li
Journal:  Acta Biochim Biophys Sin (Shanghai)       Date:  2005-06       Impact factor: 3.848

3.  Purification of beta-glucosidase from olive (Olea europaea L.) fruit tissue with specifically designed hydrophobic interaction chromatography and characterization of the purified enzyme.

Authors:  Hatibe Ertürk Kara; Selma Sinan; Yusuf Turan
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2011-04-12       Impact factor: 3.205

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Purification and properties of a stable beta-glucosidase from an extremely thermophilic anaerobic bacterium.

Authors:  M L Patchett; R M Daniel; H W Morgan
Journal:  Biochem J       Date:  1987-05-01       Impact factor: 3.857

6.  Partial purification and characterization of a soybean beta-glucosidase with high specific activity towards isoflavone conjugates.

Authors:  M C Hsieh; T L Graham
Journal:  Phytochemistry       Date:  2001-12       Impact factor: 4.072

7.  Purification and characterization of ginsenoside-beta-glucosidase from ginseng.

Authors:  C Zhang; H Yu; Y Bao; L An; F Jin
Journal:  Chem Pharm Bull (Tokyo)       Date:  2001-07       Impact factor: 1.645

8.  Characterization of a β-glucosidase from Sulfolobus solfataricus for isoflavone glycosides.

Authors:  Bi-Na Kim; Soo-Jin Yeom; Yeong-Su Kim; Deok-Kun Oh
Journal:  Biotechnol Lett       Date:  2011-09-06       Impact factor: 2.461

9.  Characterization of a thermostable extracellular beta-glucosidase with activities of exoglucanase and transglycosylation from Paecilomyces thermophila.

Authors:  Shaoqing Yang; Zhengqiang Jiang; Qiaojuan Yan; Huifang Zhu
Journal:  J Agric Food Chem       Date:  2007-12-20       Impact factor: 5.279

10.  Purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from Aspergillus oryzae.

Authors:  C Riou; J M Salmon; M J Vallier; Z Günata; P Barre
Journal:  Appl Environ Microbiol       Date:  1998-10       Impact factor: 4.792
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  1 in total

1.  Production of β-glucosidase from okara fermentation using Kluyveromyces marxianus.

Authors:  Min Su; Yang Hu; Yang Cui; Yuhua Wang; Hansong Yu; Junmei Liu; Weichang Dai; Chunhong Piao
Journal:  J Food Sci Technol       Date:  2020-07-08       Impact factor: 2.701
  1 in total

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