| Literature DB >> 26603231 |
Marcelo Andreetta Corral1, Fabiana Martins de Paula1, Maiara Gottardi1, Dirce Mary Correia Lima Meisel1, Vera Lucia Pagliusi Castilho2, Elenice Messias do Nascimento Gonçalves2, Pedro Paulo Chieffi1, Ronaldo Cesar Borges Gryschek1.
Abstract
The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.Entities:
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Year: 2015 PMID： 26603231 PMCID： PMC4660453 DOI： 10.1590/S0036-46652015000500010
Source DB: PubMed Journal: Rev Inst Med Trop Sao Paulo ISSN： 0036-4665 Impact factor: 1.846