Saeed Daneshmandi1, Ali Akbar Pourfathollah1, Mohammad Hossein Karimi2, Modjtaba Emadi-Baygi3,4. 1. Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 2. Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Department of Genetics, Shahrekord University, Shahrekord, Iran. 4. Research institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
Abstract
AIM: We provided potent dendritic cells (DCs) for induction of stronger antitumor immune responses. MATERIALS & METHODS: Using siRNA and shRNA systems, PDL-1 and PDL-2 were knocked down and then DC in vitro and in vivo properties were evaluated. RESULTS: Mild suppression of PDL-1/PDL-2 molecules was accompanied by appropriate expression of DCs co-stimulatory molecules and release of proinflammatory cytokines. In vitro T-cell engagement induced the proliferation and secretion of Th1 cytokines. Injection of DCs to a 4T1 mice model induced intratumor CD8(+) infiltrating lymphocytes, splenocytes expansion, Th1 cytokine profile shift, and a mild drift to tumor growth inhibition and mice survival. CONCLUSION: Manipulated DCs induced significant antitumor immunity, but this subject needs further evaluation in different animals.
AIM: We provided potent dendritic cells (DCs) for induction of stronger antitumor immune responses. MATERIALS & METHODS: Using siRNA and shRNA systems, PDL-1 and PDL-2 were knocked down and then DC in vitro and in vivo properties were evaluated. RESULTS: Mild suppression of PDL-1/PDL-2 molecules was accompanied by appropriate expression of DCs co-stimulatory molecules and release of proinflammatory cytokines. In vitro T-cell engagement induced the proliferation and secretion of Th1 cytokines. Injection of DCs to a 4T1 mice model induced intratumor CD8(+) infiltrating lymphocytes, splenocytes expansion, Th1 cytokine profile shift, and a mild drift to tumor growth inhibition and mice survival. CONCLUSION: Manipulated DCs induced significant antitumor immunity, but this subject needs further evaluation in different animals.
Authors: Maarten Versteven; Johan M J Van den Bergh; Elly Marcq; Evelien L J Smits; Viggo F I Van Tendeloo; Willemijn Hobo; Eva Lion Journal: Front Immunol Date: 2018-03-01 Impact factor: 7.561