Mohamed El Fadel Ousmaal1, M Carmen Martínez2, Ramaroson Andriantsitohaina3, Kahina Chabane4, Abderahim Gaceb5, Saâdia Mameri6, Jean Giaimis7, Ahsene Baz8. 1. Laboratory of Biology and Organism Physiology, University of Sciences and Technology Houari Boumediene (USTHB), Algiers, Algeria; Laboratory of Biology and Animal Physiology, ENS Kouba, Algiers, Algeria. Electronic address: ousmaal_med@hotmail.fr. 2. INSERM U1063- Stress Oxydant et Pathologies Métaboliques, Université d'Angers, France. Electronic address: carmen.martinez@univ-angers.fr. 3. INSERM U1063- Stress Oxydant et Pathologies Métaboliques, Université d'Angers, France. Electronic address: ramaroson.andriantsitohaina@univ-angers.fr. 4. Laboratory of Biology and Animal Physiology, ENS Kouba, Algiers, Algeria. Electronic address: kahinachabane@hotmail.fr. 5. INSERM U1063- Stress Oxydant et Pathologies Métaboliques, Université d'Angers, France. Electronic address: fond.bleu@hotmail.fr. 6. Laboratory of Anatomopathology, Mustapha Bacha Hospital, Algiers, Algeria. Electronic address: saadiamameri@hotmail.com. 7. UMR Qualisud- Faculty of Pharmacy, University of Montpellier I, Montpellier, France. Electronic address: jean.giaimis@univ-montp1.fr. 8. Laboratory of Biology and Animal Physiology, ENS Kouba, Algiers, Algeria. Electronic address: baznacim@hotmail.com.
Abstract
AIMS: To compare the effects of a high-energy diet (HED) with those of a low-energy diet (LED) on biochemical parameters, microparticle (MP) subpopulations and endothelial caveolin-1 (cav-1) protein expression in Psammomys obesus (P. obesus). METHODS: After 12weeks of feeding with either the HED or LED, fasting plasma glucose and lipid parameters were measured using an enzymatic colorimetric kit while serum insulin concentration was determined with radioimmunoassay kits. MP subpopulations and cav-1 protein expression were quantified using flow cytometry and western blot analysis, respectively. RESULTS: We observed that the HED caused a marked increase in lipid parameters, even in normoglycemic P. obesus. The total number of circulating MPs and the numbers of platelet-, leukocyte-, and erythrocyte-derived MPs were unaltered in the HED group. However, the HED induced increases in the numbers of monocytes/neutrophils and procoagulant MPs and a decrease in the endothelial MP levels. Cav-1β protein expression and reactive oxygen species production were increased in the vascular endothelium of HED-treated P. obesus. CONCLUSION: From these findings, it is indicated that the HED exerts deleterious effects on the vascular system by increasing the monocyte/neutrophil and procoagulant MP levels, which may lead to cav-1β protein overexpression in dyslipidemic P. obesus.
AIMS: To compare the effects of a high-energy diet (HED) with those of a low-energy diet (LED) on biochemical parameters, microparticle (MP) subpopulations and endothelial caveolin-1 (cav-1) protein expression in Psammomys obesus (P. obesus). METHODS: After 12weeks of feeding with either the HED or LED, fasting plasma glucose and lipid parameters were measured using an enzymatic colorimetric kit while serum insulin concentration was determined with radioimmunoassay kits. MP subpopulations and cav-1 protein expression were quantified using flow cytometry and western blot analysis, respectively. RESULTS: We observed that the HED caused a marked increase in lipid parameters, even in normoglycemic P. obesus. The total number of circulating MPs and the numbers of platelet-, leukocyte-, and erythrocyte-derived MPs were unaltered in the HED group. However, the HED induced increases in the numbers of monocytes/neutrophils and procoagulant MPs and a decrease in the endothelial MP levels. Cav-1β protein expression and reactive oxygen species production were increased in the vascular endothelium of HED-treated P. obesus. CONCLUSION: From these findings, it is indicated that the HED exerts deleterious effects on the vascular system by increasing the monocyte/neutrophil and procoagulant MP levels, which may lead to cav-1β protein overexpression in dyslipidemic P. obesus.