Asuman Gedikbasi1, Burak Yücel2, Oguz Arslan2, Murat Giris3, Ali Gedikbasi2, Semra Dogru Abbasoglu3. 1. a Department of Biochemistry , Istanbul Bakirkoy Sadi Konuk Research and Training Hospital , Istanbul , Turkey . 2. b Department of Obstetrics and Gynecology , Kanuni Sultan Suleyman Research and Training Research Hospital , Istanbul , Turkey , and. 3. c Department of Biochemistry , Faculty of Medicine, Istanbul University , Istanbul , Turkey.
Abstract
OBJECTIVE: To determine the changes in cervical collagen during the first trimester of pregnancy and to evaluate the collagen deficit in cases with a previous diagnosis of cervical insufficiency (CI). MATERIALS AND METHODS: Cervical punch biopsies were obtained from 66 patients divided into three groups: patients with recurrent abortions due to CI (CI group; n = 8); first-trimester abortion group (study group; n = 37), subdivided into three groups according their gestational week (<7, 7-9 and 9-12 weeks), and patients with cervical biopsy due to gynecologic reasons (control group; n = 12). Collagen quantity was determined by a biochemical method that measured the levels of hydroxyproline (HOP) in dry cervix tissue. RESULTS: The HOP concentrations were significantly higher at lower gestational ages (p = 0.001). Collagen quantity was lowest in the CI group compared with other groups (p < 0.001). CONCLUSION: This study shows collagen component of cervix decreases as pregnancy advances through the first trimester. Cervical collagen concentration is lower in women with a history of CI compared to controls who has not a history of CI.
OBJECTIVE: To determine the changes in cervical collagen during the first trimester of pregnancy and to evaluate the collagen deficit in cases with a previous diagnosis of cervical insufficiency (CI). MATERIALS AND METHODS: Cervical punch biopsies were obtained from 66 patients divided into three groups: patients with recurrent abortions due to CI (CI group; n = 8); first-trimester abortion group (study group; n = 37), subdivided into three groups according their gestational week (<7, 7-9 and 9-12 weeks), and patients with cervical biopsy due to gynecologic reasons (control group; n = 12). Collagen quantity was determined by a biochemical method that measured the levels of hydroxyproline (HOP) in dry cervix tissue. RESULTS: The HOP concentrations were significantly higher at lower gestational ages (p = 0.001). Collagen quantity was lowest in the CI group compared with other groups (p < 0.001). CONCLUSION: This study shows collagen component of cervix decreases as pregnancy advances through the first trimester. Cervical collagen concentration is lower in women with a history of CI compared to controls who has not a history of CI.
Authors: Joy Vink; Victoria Yu; Sudip Dahal; James Lohner; Conrad Stern-Asher; Mirella Mourad; George Davis; Zenghui Xue; Shuang Wang; Kristin Myers; Jan Kitajewski; Xiaowei Chen; Ronald J Wapner; Cande V Ananth; Michael Sheetz; George Gallos Journal: Reprod Sci Date: 2020-07-22 Impact factor: 2.924