| Literature DB >> 26593534 |
Shuzhen Yang1, Limei Liu1, Dongmei Li2, Huan Xia1, Xiaojun Su3, Litao Peng4, Siyi Pan1.
Abstract
Antifungal components, from poplar buds active fraction (PBAF) against Penicillium italicum, the causal agent of blue mold in citrus fruits, were identified and possible action modes were investigated. Pinocembrin, chrysin and galangin were determined as active components in PBAF, using HPLC and HPLC-MS analysis. The antifungal activity is stable at temperatures ranging from 4 °C to 100 °C and pH levels ranging from 4 to 8. In the presence of PBAF, the hyphae become shriveled, wrinkled and the cell membrane became seriously disrupted. Further investigation on cell permeability, nucleic acid content and alkaline phosphatase suggest that the cell membrane might be the target. Mycelial oxygen consumption and the respiration-related enzymatic activity of succinate dehydrogenase, malate dehydrogenase and ATPase were all inhibited by PBAF. We propose that PBAF is a potentially useful alternative for blue mold control and may act against P. italicum by interfering with respiration and disrupting the cell membrane.Entities:
Keywords: Active constituents; Mode of action; P. italicum; Poplar buds; Respiration
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Year: 2015 PMID： 26593534 DOI： 10.1016/j.foodchem.2015.09.101
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514