| Literature DB >> 26593521 |
Lifeng Wang1, Chao Chen2, Anxiang Su2, Yiyi Zhang2, Jian Yuan2, Xingrong Ju2.
Abstract
The current study aims to investigate the antioxidant activities of various extracts from defatted adlay seed meal (DASM) based on the oxygen radical absorbance capacity (ORAC) assay, peroxyl radical scavenging capacity (PSC) assay and cellular antioxidant activity (CAA) assay. Of all the fractions, the n-butanol fraction exhibited the highest antioxidant activity, followed by crude acetone extract and aqueous fractions. Of the three sub-fractions obtained by Sephadex LH-20 chromatography, sub-fraction 3 possessed the highest antioxidant activity and total phenolic content. There was a strong positive correlation between the total phenolic content and the antioxidant activity. Based on HPLC-DAD-ESI-MS/MS analysis, the most abundant phenolic acid in sub-fraction 3 of DASM was ferulic acid at 67.28 mg/g, whereas the predominant flavonoid was rutin at 41.11 mg/g. Of the major individual compounds in sub-fraction 3, p-coumaric acid exhibited the highest ORAC values, and quercetin exhibited the highest PSC values and CAA values.Entities:
Keywords: Antioxidant activity; Defatted adlay seed meal; Ferulic acid; Fractionation; Phenolic compounds; Quercetin; Rutin
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Year: 2015 PMID： 26593521 DOI： 10.1016/j.foodchem.2015.09.083
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514