Guillaume Ghislain Aubin1,2, Jean-Philippe Lavigne3,4, Béatrice Guyomarch5, Christian Dina5, François Gouin6, Didier Lepelletier1,2, Stéphane Corvec1,2. 1. Laboratoire UPRES EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France. 2. Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France. 3. INSERM U1047, Université de Montpellier, Nîmes, France. 4. Service de Microbiologie, CHU Carémeau, Nîmes University Hospital, Nîmes, France. 5. INSERM/Unité Mixte de Recherche (UMR) 1087, Institut du Thorax, Nantes, France. 6. Clinique chirurgicale orthopédique et traumatique, Nantes University Hospital, France.
Abstract
AIM: To identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). MATERIALS & METHODS: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods. RESULTS: Staphylokinase gene was associated with the occurrence of S. aureus IAI. Patients' ABO blood group phenotype was associated with IAI S. aureus genetic background. CC8 S. aureus strains produce more biofilm than others and carry particular alleles of bbp gene. CONCLUSION: This study identifies some predictive markers for S. aureus IAI.
AIM: To identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). MATERIALS & METHODS: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods. RESULTS: Staphylokinase gene was associated with the occurrence of S. aureus IAI. Patients' ABO blood group phenotype was associated with IAI S. aureus genetic background. CC8 S. aureus strains produce more biofilm than others and carry particular alleles of bbp gene. CONCLUSION: This study identifies some predictive markers for S. aureus IAI.
Entities:
Keywords:
Staphylococcus aureus; biofilm; blood group; bone and joint infection; bone binding sialoprotein; microarrays; staphylokinase