OBJECTIVE: To analyze the frequency of plasmacytoid dendritic cells (pDCs) in pregnancy, especially their subsets, by flow cytometry in each trimester and in the postpartum period. STUDY DESIGN: Peripheral blood was collected from pregnant women in each trimester and from non-pregnant women. Serum hormones were detected by ELISA, while pDCs and their subsets were detected by flow cytometer. Then correlation between them was further analyzed. RESULTS: Both pDCs and their subsets declined as pregnancy progressed. Further experiments indicate that estradiol and progesterone are significantly negatively correlated with this change. CONCLUSION: Our findings increase the knowledge of pDC subsets in healthy pregnant conditions.
OBJECTIVE: To analyze the frequency of plasmacytoid dendritic cells (pDCs) in pregnancy, especially their subsets, by flow cytometry in each trimester and in the postpartum period. STUDY DESIGN: Peripheral blood was collected from pregnant women in each trimester and from non-pregnant women. Serum hormones were detected by ELISA, while pDCs and their subsets were detected by flow cytometer. Then correlation between them was further analyzed. RESULTS: Both pDCs and their subsets declined as pregnancy progressed. Further experiments indicate that estradiol and progesterone are significantly negatively correlated with this change. CONCLUSION: Our findings increase the knowledge of pDC subsets in healthy pregnant conditions.
Authors: Elizabeth A N Wastnedge; Rebecca M Reynolds; Sara R van Boeckel; Sarah J Stock; Fiona C Denison; Jacqueline A Maybin; Hilary O D Critchley Journal: Physiol Rev Date: 2020-09-24 Impact factor: 37.312