Exposure to difenoconazole causes changes of thyroid hormone and gene expression levels in zebrafish larvae.

Difenoconazole was believed to induce a large suite of symptoms during zebrafish development, but little is known about the negative invisible effect known as endocrine disruption. In this study, zebrafish (Danio rerio) embryos were exposed to various concentrations of difenoconazole from fertilization to 120 h post-fertilization (hpf), and the whole body content of thyroid hormone and gene transcription in the hypothalamic-pituitary-thyroid (HPT) axis were investigated. Results showed thyroxine (T4) levels were significantly decreased, while triiodothyronine (T3) concentrations were not changed. Moreover, the mRNA transcription of corticotrophin-releasing hormone (crh), thyroid-stimulating hormone (tshβ), transthyretin (ttr), thyronine deiodinase (dio1 and dio2), uridine diphosphate glucuronosyltransferase (ugt1ab) in the HPT axis were significantly up-regulated, but the transcriptions of thyroglobulin (tg), sodium/iodide symporter (nis) and thyroid hormone receptors trβ were not changed. The overall results showed that exposure to difenoconazole could alter thyroid hormone levels and gene transcription in zebrafish larvae, indicating thyroid endocrine disruption.