Giannicola Iannella1, Giovanni Di Nardo2, Rocco Plateroti3, Paolo Rossi4, Andrea Maria Plateroti5, Paola Mariani6, Giuseppe Magliulo7. 1. Organi di Senso Department, University "La Sapienza", Viale del Policlinico, 151, 00161 Rome, Italy. Electronic address: giannicolaiannella@hotmail.it. 2. Department of Pediatrics, University "La Sapienza", Viale del Policlinico, 151, 00161 Rome, Italy. Electronic address: giovanni.dinardo@uniroma1.it. 3. Organi di Senso Department, University "La Sapienza", Viale del Policlinico, 151, 00161 Rome, Italy. Electronic address: rocco.plateroti@uniroma1.it. 4. Department of Pediatrics, University "La Sapienza", Viale del Policlinico, 151, 00161 Rome, Italy. Electronic address: paolo.rossi@uniroma1.it. 5. Organi di Senso Department, University "La Sapienza", Viale del Policlinico, 151, 00161 Rome, Italy. Electronic address: andrea.plateroti@gmail.com. 6. Department of General and specialized surgery Paride Stefanini, University "La Sapienza", Viale del Policlinico, 151, 00161 Rome, Italy. Electronic address: paola.mariani@uniroma1.it. 7. Organi di Senso Department, University "La Sapienza", Viale del Policlinico, 151, 00161 Rome, Italy. Electronic address: giuseppemagliuloorl@yahoo.com.
Abstract
OBJECTIVES: Numerous investigations postulated that laryngopharyngeal reflux (LPR) is implicated in the pathogenesis of various upper airway inflammatory diseases as sinusitis or dacryostenosis. The presence of pepsin in tears might be confirmed the presuntive hypothesis of the arrival in the nasolacrimal ducts and precorneal tears film through the laryngopharyngeal reflux of either gastric acid or stomach secretions (pepsin) with inflammatory potentialities. The aim of this preliminary study was to identify the presence or absence of pepsin in the tears collected from children with a high suspicion of LPR who underwent 24-h pH (MII-pH) monitoring to confirm the disease. METHODS: This study enrolled 20 patients suffering from symptoms of laryngopharyngeal reflux that underwent 24-h multichannel intraluminal impedance (MII)-pH monitoring to confirm the disease. The findings of the study group were compared with those of a control group of patients with negative pH monitoring. The quantitative analysis of human pepsin concentration in the tear samples was performed by ELISA method in both groups. RESULTS: Four children (20%) of the study group showed pepsin in the tears. All of the subjects belonging to the control group were negative for its presence. No difference differences in the total number of reflux episodes and the number of weakly basic reflux in the pepsin positive patients vs. pepsin negative children were present. CONCLUSIONS: 20% of the children with diagnosed LPR showed pepsin in the tears. Our specific investigation might provide information regarding sinusitis or dacryostenosis.
OBJECTIVES: Numerous investigations postulated that laryngopharyngeal reflux (LPR) is implicated in the pathogenesis of various upper airway inflammatory diseases as sinusitis or dacryostenosis. The presence of pepsin in tears might be confirmed the presuntive hypothesis of the arrival in the nasolacrimal ducts and precorneal tears film through the laryngopharyngeal reflux of either gastric acid or stomach secretions (pepsin) with inflammatory potentialities. The aim of this preliminary study was to identify the presence or absence of pepsin in the tears collected from children with a high suspicion of LPR who underwent 24-h pH (MII-pH) monitoring to confirm the disease. METHODS: This study enrolled 20 patients suffering from symptoms of laryngopharyngeal reflux that underwent 24-h multichannel intraluminal impedance (MII)-pH monitoring to confirm the disease. The findings of the study group were compared with those of a control group of patients with negative pH monitoring. The quantitative analysis of human pepsin concentration in the tear samples was performed by ELISA method in both groups. RESULTS: Four children (20%) of the study group showed pepsin in the tears. All of the subjects belonging to the control group were negative for its presence. No difference differences in the total number of reflux episodes and the number of weakly basic reflux in the pepsin positive patients vs. pepsin negative children were present. CONCLUSIONS: 20% of the children with diagnosed LPR showed pepsin in the tears. Our specific investigation might provide information regarding sinusitis or dacryostenosis.
Authors: Giannicola Iannella; Claudio Vicini; Antonella Polimeni; Antonio Greco; Riccardo Gobbi; Filippo Montevecchi; Andrea De Vito; Giuseppe Meccariello; Giovanni Cammaroto; Giovanni D'Agostino; Annalisa Pace; Raffaella Cascella; Marco Brunori; Cristina Anna Maria Lo Iacono; Stefano Pelucchi; Giuseppe Magliulo Journal: Int J Environ Res Public Health Date: 2019-06-10 Impact factor: 3.390
Authors: Peter William Dettmar; Andrew David Woodcock; Matthew David Wilcox; Peter Ian Chater; Jeffrey Peter Pearson Journal: Aliment Pharmacol Ther Date: 2019-05 Impact factor: 8.171
Authors: Rocco Plateroti; Marta Sacchetti; Giuseppe Magliulo; Andrea Maria Plateroti; Annalisa Pace; Antonietta Moramarco; Alessandro Lambiase; Alice Bruscolini Journal: Life (Basel) Date: 2020-09-15