Hyoung Shin Lee1, Sung Won Kim1, Chulho Oak2, Yeh-Chan Ahn3, Hyun Wook Kang3, Bong Kwon Chun4, Kang Dae Lee5. 1. Department of Otolaryngology-Head and Neck Surgery, Kosin University College of Medicine, Busan, Korea; Innovative Biomedical Technology Research Center, College of Medicine, Kosin University, Busan, Korea. 2. Innovative Biomedical Technology Research Center, College of Medicine, Kosin University, Busan, Korea; Department of Internal Medicine, Kosin University College of Medicine, Busan, Korea. Electronic address: oaks70@hanmail.net. 3. Innovative Biomedical Technology Research Center, College of Medicine, Kosin University, Busan, Korea; Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University, Busan, South Korea. 4. Department of Pathology, Kosin University College of Medicine, Busan, Korea. 5. Department of Otolaryngology-Head and Neck Surgery, Kosin University College of Medicine, Busan, Korea.
Abstract
OBJECTIVE: Animal model of tracheal stenosis based on pathophysiology of prolonged endotracheal intubation has been rarely reported. We sought to verify the feasibility of inducing an animal model of tracheal stenosis by segmented endotracheal tube insertion in the New Zealand white rabbit model. METHODS: Tracheal stenosis was induced by inserting a segmented endotracheal tube of 1.5cm length which was wrapped with a commercialized absorbable hemostat in 15 New Zealand white rabbits, while sham surgery controls (n=3) underwent tracheotomy and direct closure of tracheal exposure. The tube was removed transorally, 1 week after tube insertion. All rabbits were evaluated endoscopically at 1 week, 2 weeks and 3 weeks after the tube insertion. The rabbits were sacrificed 3 weeks after the surgery, and the excised tissue of trachea was processed along with the procedure of standard hematoxylin eosin staining and observed under a microscope. RESULTS: Tracheal stenosis was induced in all rabbits (range 32-84% stenosis) with no death of rabbits during the study. The histological features of tracheal stenosis demonstrated thickening and fibrosis of lamina propria and submucosa with relatively intact cartilage framework. CONCLUSIONS: We developed a rabbit model of tracheal stenosis induced by endotracheal intubation using a segmented tracheal tube. Since the model is based on the physiologic condition of prolonged endotracheal intubation, it may be used in variable studies related to tracheal stenosis.
OBJECTIVE: Animal model of tracheal stenosis based on pathophysiology of prolonged endotracheal intubation has been rarely reported. We sought to verify the feasibility of inducing an animal model of tracheal stenosis by segmented endotracheal tube insertion in the New Zealand white rabbit model. METHODS:Tracheal stenosis was induced by inserting a segmented endotracheal tube of 1.5cm length which was wrapped with a commercialized absorbable hemostat in 15 New Zealand white rabbits, while sham surgery controls (n=3) underwent tracheotomy and direct closure of tracheal exposure. The tube was removed transorally, 1 week after tube insertion. All rabbits were evaluated endoscopically at 1 week, 2 weeks and 3 weeks after the tube insertion. The rabbits were sacrificed 3 weeks after the surgery, and the excised tissue of trachea was processed along with the procedure of standard hematoxylin eosin staining and observed under a microscope. RESULTS:Tracheal stenosis was induced in all rabbits (range 32-84% stenosis) with no death of rabbits during the study. The histological features of tracheal stenosis demonstrated thickening and fibrosis of lamina propria and submucosa with relatively intact cartilage framework. CONCLUSIONS: We developed a rabbit model of tracheal stenosis induced by endotracheal intubation using a segmented tracheal tube. Since the model is based on the physiologic condition of prolonged endotracheal intubation, it may be used in variable studies related to tracheal stenosis.
Authors: Mary Patrice Eastwood; Luc Joyeux; Savitree Pranpanus; Johannes Van der Merwe; Eric Verbeken; Stephanie De Vleeschauwer; Ghislaine Gayan-Ramirez; Jan Deprest Journal: PLoS One Date: 2017-03-30 Impact factor: 3.240
Authors: Gunpreet Oberoi; M C Eberspächer-Schweda; Sepideh Hatamikia; Markus Königshofer; Doris Baumgartner; Anne-Margarethe Kramer; Peter Schaffarich; Hermann Agis; Francesco Moscato; Ewald Unger Journal: Front Vet Sci Date: 2020-11-27