Mariane Avante Ferraz1, Luanna Munhoz Zabaglia1, Weendelly Nayara Pereira1, Wilson Aparecido Orcini1, Roger Willian de Labio2, Agostinho Caleman Neto2, Fernanda Wisnieski3, Danielle Queiroz Calcagno3, Leonardo Caires Santos3, Paulo Pimentel Assumpção4, Rommel Rodriguez Burbano5, Ricardo Artigiani6, Marilia de Arruda Cardoso Smith3, Spencer Luiz Marques Payão1, Lucas T Rasmussen7. 1. Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil. 2. Departamento de Genética e Biologia Molecular, Hemocentro, Faculdade de Medicina de Marília, Rua Monte Carmelo, 800, Marília, 17519050, Brazil. 3. Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil. 4. Núcleo de Pesquisa em Oncologia, Hospital João de Barros Barreto, Universidade Federal do Pará, Avenida Mundurucus, 4487, Belém, 66073000, Brazil. 5. Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Correia, 01, Belém, 66075110, Brazil. 6. Departamento de Patologia, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023000, Brazil. 7. Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil. lucasrasmussen@gmail.com.
Abstract
PURPOSE: Gastritis caused by infection with Helicobacter pylori is characterized by chronic inflammation and damage in gastric tissue, which is a main risk factor for gastric cancer. Associated with H. pylori, the TP53 gene tumor suppressor and the cell adhesion glycoprotein epithelial cadherin develop a relevant role in the integrity and carcinogenesis of the epithelium. We aimed to detection of H. pylori and its main virulence markers and measured the messenger RNA (mRNA) expression levels of E-cadherin and TP53 genes. METHODS: The detection of H. pylori and its virulence markers, as well as the mRNA expression levels of E-cadherin and TP53 genes, were obtained for 161 samples of gastric biopsies including 37 with normal gastric tissue, 70 with gastritis, 24 from neoplastic tissue, and 27 from adjacent non-neoplastic by means of a quantitative real-time polymerase chain reaction. RESULTS: The mRNA expression levels of E-cadherin and TP53 were found to be decreased in patients with gastritis, independently of H. pylori infection. In samples from gastric patients, the neoplastic tissue showed an accentuated decrease of expression; on the other hand, the expression of E-cadherin was normal in adjacent non-neoplastic. CONCLUSIONS: No evidence was found of the involvement of the cagA and vacA genes in the decreased expression of E-cadherin and TP53. The process of carcinogenesis is complex, and the decrease of the E-cadherin gene expression and TP53 gene expression appears to contribute significantly.
PURPOSE:Gastritis caused by infection with Helicobacter pylori is characterized by chronic inflammation and damage in gastric tissue, which is a main risk factor for gastric cancer. Associated with H. pylori, the TP53 gene tumor suppressor and the cell adhesion glycoprotein epithelial cadherin develop a relevant role in the integrity and carcinogenesis of the epithelium. We aimed to detection of H. pylori and its main virulence markers and measured the messenger RNA (mRNA) expression levels of E-cadherin and TP53 genes. METHODS: The detection of H. pylori and its virulence markers, as well as the mRNA expression levels of E-cadherin and TP53 genes, were obtained for 161 samples of gastric biopsies including 37 with normal gastric tissue, 70 with gastritis, 24 from neoplastic tissue, and 27 from adjacent non-neoplastic by means of a quantitative real-time polymerase chain reaction. RESULTS: The mRNA expression levels of E-cadherin and TP53 were found to be decreased in patients with gastritis, independently of H. pyloriinfection. In samples from gastric patients, the neoplastic tissue showed an accentuated decrease of expression; on the other hand, the expression of E-cadherin was normal in adjacent non-neoplastic. CONCLUSIONS: No evidence was found of the involvement of the cagA and vacA genes in the decreased expression of E-cadherin and TP53. The process of carcinogenesis is complex, and the decrease of the E-cadherin gene expression and TP53 gene expression appears to contribute significantly.
Authors: L J van Doorn; C Figueiredo; R Rossau; G Jannes; M van Asbroek; J C Sousa; F Carneiro; W G Quint Journal: J Clin Microbiol Date: 1998-05 Impact factor: 5.948
Authors: N Murata-Kamiya; Y Kurashima; Y Teishikata; Y Yamahashi; Y Saito; H Higashi; H Aburatani; T Akiyama; R M Peek; T Azuma; M Hatakeyama Journal: Oncogene Date: 2007-01-22 Impact factor: 9.867
Authors: Krzysztof Kułak; Marcin Bobiński; Grzegorz Polak; Barbara Jodłowska Jedrych; Jan Kotarski; Wiesława Bednarek Journal: Ginekol Pol Date: 2014-08 Impact factor: 1.232