Andrzej Pawlik1, Daniel Kotrych2, Edyta Paczkowska3, Dorota Roginska3, Violetta Dziedziejko4, Krzysztof Safranow4, Bogusław Machalinski3. 1. Department of Physiology, Pomeranian Medical University, Szczecin, Poland. Electronic address: pawand@poczta.onet.pl. 2. Department of Orthopaedics, Traumatology and Orthopaedic Oncology, Pomeranian Medical University, Szczecin, Poland. 3. Department of General Pathology, Pomeranian Medical University, Szczecin, Poland. 4. Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland.
Abstract
OBJECTIVE: Allograft inflammatory factor-1 (AIF-1) is a cytoplasmic protein expressed in various human cells such as monocyte/macrophages and activated T lymphocytes. A recent study showed that AIF-1 is strongly expressed in infiltrating mononuclear cells and synovial fibroblasts in rheumatoid arthritis and that AIF-1 induces the proliferation of cultured synovial cells. In this study we analysed the expression of AIF-1 in peripheral blood monocytes and synovial membranes from patients with rheumatoid arthritis (RA). METHODS: We examined 71 patients with rheumatoid arthritis and 25 control subjects. RESULTS: Using flow cytometry we found significantly increased numbers of circulating AIF-1(+) monocytes in peripheral blood from RA patients compared with controls. Moreover, there were statistically significant positive correlations between AIF-1(+) monocytes, DAS28 and the Sharp erosion score. Immunofluorescence staining showed strong expression of AIF-1 by infiltrating mononuclear cells - predominantly macrophages in RA synovial tissues - compared with tissues derived from joints affected by osteoarthritis. CONCLUSION: The results of this study suggest that AIF-1 may be associated with the pathogenesis of RA and may be a novel cytokine involved in the immunological process underlying RA.
OBJECTIVE:Allograft inflammatory factor-1 (AIF-1) is a cytoplasmic protein expressed in various human cells such as monocyte/macrophages and activated T lymphocytes. A recent study showed that AIF-1 is strongly expressed in infiltrating mononuclear cells and synovial fibroblasts in rheumatoid arthritis and that AIF-1 induces the proliferation of cultured synovial cells. In this study we analysed the expression of AIF-1 in peripheral blood monocytes and synovial membranes from patients with rheumatoid arthritis (RA). METHODS: We examined 71 patients with rheumatoid arthritis and 25 control subjects. RESULTS: Using flow cytometry we found significantly increased numbers of circulating AIF-1(+) monocytes in peripheral blood from RApatients compared with controls. Moreover, there were statistically significant positive correlations between AIF-1(+) monocytes, DAS28 and the Sharp erosion score. Immunofluorescence staining showed strong expression of AIF-1 by infiltrating mononuclear cells - predominantly macrophages in RA synovial tissues - compared with tissues derived from joints affected by osteoarthritis. CONCLUSION: The results of this study suggest that AIF-1 may be associated with the pathogenesis of RA and may be a novel cytokine involved in the immunological process underlying RA.
Authors: Kathleen M Donovan; Mariah R Leidinger; Logan P McQuillen; J Adam Goeken; Christine M Hogan; Sailesh C Harwani; Heather A Flaherty; David K Meyerholz Journal: Comp Med Date: 2018-09-18 Impact factor: 0.982
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