Literature DB >> 26585132

Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two-Dimensional Liquid Chromatography and Tandem Mass Spectrometry.

Eduardo A Callegari1.   

Abstract

Shotgun (gel-free) proteomics is a useful approach to perform identification and relative quantification of protein in complex mixtures such as tissue homogenates, biological fluids, cell lysates, and extracellular proteins. Incorporation of separative and analytical techniques such as two-dimensional liquid chromatography at nanoscale (2D-nanoLC) coupled to tandem mass spectrometry (MS/MS analysis) into the shotgun protocol provides an excellent strategy. This chapter describes the application of the shotgun proteomics protocol to evaluate the identity and expression analysis of proteins from rat uterus after estrogen (ethinylestradiol) treatment. The steps of the protocol involve sample preparation (digestion), 2D-nanoLC-MS/MS analysis, and shotgun proteomics analysis including bioinformatics tools for data conversion, organization, and interpretation.

Entities:  

Keywords:  Estrogen; Liquid chromatography; Mass spectrometry; Peptides; Proteins; Proteomics; Rat; Uterus; “Shotgun” proteomics

Mesh：

Substances：

Year:  2016        PMID： 26585132      PMCID： PMC5364499          DOI： 10.1007/978-1-4939-3127-9_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  Effects of the position of internal histidine residues on the collision-induced fragmentation of triply protonated tryptic peptides.

Authors:  B B Willard; M Kinter
Journal:  J Am Soc Mass Spectrom       Date:  2001-12       Impact factor: 3.109

2.  Mass spectrometric identification of novel posttranslational modification sites in Huntingtin.

Authors:  Gaofeng Dong; Eduardo Callegari; Christian J Gloeckner; Marius Ueffing; Hongmin Wang
Journal:  Proteomics       Date:  2012-06       Impact factor: 3.984

3.  Two-dimensional separation of peptides using RP-RP-HPLC system with different pH in first and second separation dimensions.

Authors:  Martin Gilar; Petra Olivova; Amy E Daly; John C Gebler
Journal:  J Sep Sci       Date:  2005-09       Impact factor: 3.645

Review 4.  Tools for label-free peptide quantification.

Authors:  Sven Nahnsen; Chris Bielow; Knut Reinert; Oliver Kohlbacher
Journal:  Mol Cell Proteomics       Date:  2012-12-17       Impact factor: 5.911

5.  Differential protein expression profiles in estrogen receptor-positive and -negative breast cancer tissues using label-free quantitative proteomics.

Authors:  Karim Rezaul; Jay Kumar Thumar; Deborah H Lundgren; Jimmy K Eng; Kevin P Claffey; Lori Wilson; David K Han
Journal:  Genes Cancer       Date:  2010-03

6.  Ceruloplasmin gene expression in the rat uterus.

Authors:  T Thomas; A Macpherson; P Rogers
Journal:  Biochim Biophys Acta       Date:  1995-03-14

7.  Label-free electrical determination of trypsin activity by a silicon-on-insulator based thin film resistor.

Authors:  Petra A Neff; Andreas Serr; Bernhard K Wunderlich; Andreas R Bausch
Journal:  Chemphyschem       Date:  2007-10-08       Impact factor: 3.102

8.  Eosinophil accumulation in rat uterus following estradiol administration is modulated by laminin and its integrin receptors.

Authors:  M L Katayama; M H Federico; R R Brentani; M M Brentani
Journal:  Cell Adhes Commun       Date:  1998-07

9.  Inhibition of estrogen-mediated uterine gene expression responses by dioxin.

Authors:  Darrell R Boverhof; Lyle D Burgoon; Kurt J Williams; Timothy R Zacharewski
Journal:  Mol Pharmacol       Date:  2007-10-17       Impact factor: 4.436

10.  Rapid label-free identification of estrogen-induced differential protein expression in vivo from mouse brain and uterine tissue.

Authors:  Laszlo Prokai; Stanley M Stevens; Navin Rauniyar; Vien Nguyen
Journal:  J Proteome Res       Date:  2009-08       Impact factor: 4.466
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  3 in total

1.  Metalloproteomics analysis in human mammary cell lines treated with inorganic mercury.

Authors:  Mariángeles Ávila Maniero; Rodolfo G Wuilloud; Eduardo A Callegari; Patricia N Smichowski; Mariel A Fanelli
Journal:  J Trace Elem Med Biol       Date:  2019-11-23       Impact factor: 3.849

2.  Corncob and sugar beet pulp induce specific sets of lignocellulolytic enzymes in Penicillium purpurogenum.

Authors:  Wladimir Mardones; Eduardo Callegari; Jaime Eyzaguirre
Journal:  Mycology       Date:  2018-09-11

3.  Proteomics Complementation of the Rat Uterotrophic Assay for Estrogenic Endocrine Disruptors: A Roadmap of Advancing High Resolution Mass Spectrometry-Based Shotgun Survey to Targeted Biomarker Quantifications.

Authors:  Laszlo Prokai; Fatima Rahlouni; Khadiza Zaman; Vien Nguyen; Katalin Prokai-Tatrai
Journal:  Int J Mol Sci       Date:  2021-02-08       Impact factor: 5.923
  3 in total

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