Literature DB >> 2658475

Luciferase cDNA from Japanese firefly, Luciola cruciata: cloning, structure and expression in Escherichia coli.

H Tatsumi1, T Masuda, N Kajiyama, E Nakano.   

Abstract

We have cloned the cDNA for luciferase from lantern poly(A)+ RNA of a Japanese firefly, Luciola cruciata (Genji botaru in Japanese). This cDNA directed the synthesis of enzymatically active luciferase under the control of the lac promoter in Escherichia coli. The amino acid sequence predicted from the cDNA sequence shows that Genji firefly luciferase consists of 548 amino acids and has a molecular weight of 60,024. Considerable sequence homology was found upon the comparison of the Genji and North American firefly luciferases.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2658475     DOI： 10.1002/bio.1170030208

Source DB:  PubMed          Journal:  J Biolumin Chemilumin        ISSN： 0884-3996


  7 in total

1.  Engineering a bioluminescent indicator for cyclic AMP-dependent protein kinase.

Authors:  G B Sala-Newby; A K Campbell
Journal:  Biochem J       Date:  1991-11-01       Impact factor: 3.857

2.  Novel antibiotic susceptibility tests by the ATP-bioluminescence method using filamentous cell treatment.

Authors:  N Hattori; M O Nakajima; K O'Hara; T Sawai
Journal:  Antimicrob Agents Chemother       Date:  1998-06       Impact factor: 5.191

3.  Sequence and biochemical similarities between the luciferases of the glow-worm Lampyris noctiluca and the firefly Photinus pyralis.

Authors:  G B Sala-Newby; C M Thomson; A K Campbell
Journal:  Biochem J       Date:  1996-02-01       Impact factor: 3.857

4.  The proton and metal binding sites responsible for the pH-dependent green-red bioluminescence color tuning in firefly luciferases.

Authors:  Vadim R Viviani; Gabriele V M Gabriel; Vanessa R Bevilaqua; A F Simões; T Hirano; P S Lopes-de-Oliveira
Journal:  Sci Rep       Date:  2018-12-04       Impact factor: 4.379

5.  Phrixotrix luciferase and 6'-aminoluciferins reveal a larger luciferin phenolate binding site and provide novel far-red combinations for bioimaging purposes.

Authors:  V R Bevilaqua; T Matsuhashi; G Oliveira; P S L Oliveira; T Hirano; V R Viviani
Journal:  Sci Rep       Date:  2019-06-21       Impact factor: 4.379

6.  Resurrecting the ancient glow of the fireflies.

Authors:  Y Oba; K Konishi; D Yano; H Shibata; D Kato; T Shirai
Journal:  Sci Adv       Date:  2020-12-02       Impact factor: 14.136

7.  A Very Bright Far-Red Bioluminescence Emitting Combination Based on Engineered Railroad Worm Luciferase and 6'-Amino-Analogs for Bioimaging Purposes.

Authors:  Vadim R Viviani; Vanessa R Bevilaqua; Daniel R de Souza; Gabriel F Pelentir; Michio Kakiuchi; Takashi Hirano
Journal:  Int J Mol Sci       Date:  2020-12-30       Impact factor: 5.923
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.