Nidhi Bhargava1, Sukhvinder P Singh2, Anupam Sharma3, Prince Sharma4, Neena Capalash1. 1. Department of Biotechnology, Panjab University, Chandigarh 160014, India. 2. NSW Department of Primary Industries, Central Coast Primary Industries Centre, Locked Bag 26, Gosford NSW 2250, Australia. 3. University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India. 4. Department of Microbiology, Panjab University, Chandigarh 160014, India.
Abstract
AIM: To develop an alternative quorum quenching therapy against multidrug-resistant Acinetobacter baumannii. METHODS & RESULTS: Activity-guided partially purified fraction (F1) from Glycyrrhiza glabra significantly (p < 0.05) reduced quorum sensing regulated virulence factors of A. baumannii viz. motility, biofilm formation and production of antioxidant enzymes. Mechanistically, F1 downregulated the expression of autoinducer synthase gene, abaI, and consequently reduced (92%) the production of 3-OH-C12-HSL as determined by ESI-MS. Q-TOF and Q-TRAP analyses suggested the presence of flavonoids viz. licoricone, glycyrin and glyzarin as the active ingredients. CONCLUSION: This is the first report on quorum quenching activity of G. glabra linked to its flavonoids that downregulated the expression of abaI and attenuated quorum sensing regulated virulence of A. baumannii.
AIM: To develop an alternative quorum quenching therapy against multidrug-resistant Acinetobacter baumannii. METHODS & RESULTS: Activity-guided partially purified fraction (F1) from Glycyrrhiza glabra significantly (p < 0.05) reduced quorum sensing regulated virulence factors of A. baumannii viz. motility, biofilm formation and production of antioxidant enzymes. Mechanistically, F1 downregulated the expression of autoinducer synthase gene, abaI, and consequently reduced (92%) the production of 3-OH-C12-HSL as determined by ESI-MS. Q-TOF and Q-TRAP analyses suggested the presence of flavonoids viz. licoricone, glycyrin and glyzarin as the active ingredients. CONCLUSION: This is the first report on quorum quenching activity of G. glabra linked to its flavonoids that downregulated the expression of abaI and attenuated quorum sensing regulated virulence of A. baumannii.
Authors: Lun K Tsou; María Lara-Tejero; Jordan RoseFigura; Zhenrun J Zhang; Yen-Chih Wang; Jacob S Yount; Matthew Lefebre; Paul D Dossa; Junya Kato; Fulan Guan; Wing Lam; Yung-Chi Cheng; Jorge E Galán; Howard C Hang Journal: J Am Chem Soc Date: 2016-02-16 Impact factor: 15.419
Authors: Katrine Qvortrup; Louise Dahl Hultqvist; Martin Nilsson; Tim Holm Jakobsen; Charlotte Uldahl Jansen; Jesper Uhd; Jens Bo Andersen; Thomas E Nielsen; Michael Givskov; Tim Tolker-Nielsen Journal: Front Chem Date: 2019-11-01 Impact factor: 5.221