Marcos Fernando Xisto Braga Cavalcanti1,2, Durvanei A Maria3, Natalia de Isla2, Ernesto Cesar Pinto Leal-Junior4, Jon Joensen5, Jan Magnus Bjordal6, Rodrigo A M B Lopes-Martins7, Francesca Diomede8, Oriana Trubiani8, Lucio Frigo1. 1. 1 Postgraduate Department, Cruzeiro do Sul University São Paulo , SP, Brasil . 2. 2 UMR-7365, Faculté de Médecine, CNRS-Université de Lorraine , Vandoeuvre-lés-Nancy, France . 3. 3 Biochemistry and Biophysics Laboratory of Institute Butantan São Paulo , SP, Brasil . 4. 4 Center for Research and Innovation in Laser , São Paulo, SP, Brasil . 5. 5 Institute for Physiotherapy, Bergen University College , Bergen Norway. 6. 6 Phisiotherapy Research Group, Department of Global and Public Health, University of Bergen , Bergen Norway. 7. 7 Biomedicine Engineering, Mogi das Cruzes University , Mogi das Cruzes, SP, Brasil . 8. 8 Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University , Chieti, Italy .
Abstract
OBJECTIVE: The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells. BACKGROUND DATA: Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation. METHODS: Low-level laser (wavelength, 660 nm; power output, 50 mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production). RESULTS: The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12 J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8 J/cm(2). CONCLUSIONS: The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
OBJECTIVE: The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells. BACKGROUND DATA: Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation. METHODS: Low-level laser (wavelength, 660 nm; power output, 50 mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production). RESULTS: The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12 J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8 J/cm(2). CONCLUSIONS: The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
Authors: Valéria Ferreira-Silva; Fernando L Primo; Munira M A Baqui; Danielle A R Magalhães; Maristela D Orellana; Andrielle Castilho-Fernandes; Mario C Cruz; Niehls O S Câmara; Dimas T Covas; Antonio C Tedesco Journal: Stem Cell Rev Rep Date: 2018-08 Impact factor: 5.739
Authors: Guilherme Aparecido Monteiro Duque da Fonseca; Marcos Fernando Xisto Braga Cavalcanti; José Daniel de Souza Maior; Juliana da Silva Pereira; Leandro Augusto Pinto; Murilo Matias; Lucio Frigo Journal: Lasers Med Sci Date: 2022-01-14 Impact factor: 3.161