Andréanne Michaud1, Sofia Laforest1, Mélissa Pelletier2, Mélanie Nadeau2, Serge Simard2, Marleen Daris2, Mathieu Lebœuf2, Hubert Vidal2, Alain Géloën2, André Tchernof3. 1. Endocrinology and NephrologyCHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), Quebec City, Quebec, Canada, G1V 4G2School of NutritionLaval University, Quebec City, Quebec, CanadaInstitut universitaire de cardiologie et de pneumologie de QuébecQuebec City, Quebec, CanadaGynecology UnitCHU de Québec-Laval University, Quebec City, Quebec, CanadaUniversity of LyonCARMEN INSERM U1060, INSA-Lyon, F-69621, Lyon, France Endocrinology and NephrologyCHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), Quebec City, Quebec, Canada, G1V 4G2School of NutritionLaval University, Quebec City, Quebec, CanadaInstitut universitaire de cardiologie et de pneumologie de QuébecQuebec City, Quebec, CanadaGynecology UnitCHU de Québec-Laval University, Quebec City, Quebec, CanadaUniversity of LyonCARMEN INSERM U1060, INSA-Lyon, F-69621, Lyon, France Endocrinology and NephrologyCHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), Quebec City, Quebec, Canada, G1V 4G2School of NutritionLaval University, Quebec City, Quebec, CanadaInstitut universitaire de cardiologie et de pneumologie de QuébecQuebec City, Quebec, CanadaGynecology UnitCHU de Québec-Laval University, Quebec City, Quebec, CanadaUniversity of LyonCARMEN INSERM U1060, INSA-Lyon, F-69621, Lyon, France. 2. Endocrinology and NephrologyCHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), Quebec City, Quebec, Canada, G1V 4G2School of NutritionLaval University, Quebec City, Quebec, CanadaInstitut universitaire de cardiologie et de pneumologie de QuébecQuebec City, Quebec, CanadaGynecology UnitCHU de Québec-Laval University, Quebec City, Quebec, CanadaUniversity of LyonCARMEN INSERM U1060, INSA-Lyon, F-69621, Lyon, France. 3. Endocrinology and NephrologyCHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), Quebec City, Quebec, Canada, G1V 4G2School of NutritionLaval University, Quebec City, Quebec, CanadaInstitut universitaire de cardiologie et de pneumologie de QuébecQuebec City, Quebec, CanadaGynecology UnitCHU de Québec-Laval University, Quebec City, Quebec, CanadaUniversity of LyonCARMEN INSERM U1060, INSA-Lyon, F-69621, Lyon, France Endocrinology and NephrologyCHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), Quebec City, Quebec, Canada, G1V 4G2School of NutritionLaval University, Quebec City, Quebec, CanadaInstitut universitaire de cardiologie et de pneumologie de QuébecQuebec City, Quebec, CanadaGynecology UnitCHU de Québec-Laval University, Quebec City, Quebec, CanadaUniversity of LyonCARMEN INSERM U1060, INSA-Lyon, F-69621, Lyon, France Endocrinology and NephrologyCHU de Quebec-Laval University, 2705 Laurier Blvd. (R-4779), Quebec City, Quebec, Canada, G1V 4G2School of NutritionLaval University, Quebec City, Quebec, CanadaInstitut universitaire de cardiologie et de pneumologie de QuébecQuebec City, Quebec, CanadaGynecology UnitCHU de Québec-Laval University, Quebec City, Quebec, CanadaUniversity of LyonCARMEN INSERM U1060, INSA-Lyon, F-69621, Lyon, France andre.tchernof@crchudequebec.ulaval.ca.
Abstract
BACKGROUND: Visceral obesity is independently related to numerous cardiometabolic alterations, with adipose tissue dysfunction as a central feature. OBJECTIVE: To examine whether omental (OM) and subcutaneous (SC) adipocyte size populations in women relate to visceral obesity, cardiometabolic risk factors and adipocyte lipolysis independent of total adiposity. DESIGN AND METHODS: OM and SC fat samples were obtained during gynecological surgery in 60 women (mean age, 46.1±5.9 years; mean BMI, 27.1±4.5 kg/m² (range, 20.3-41. kg/m²)). Fresh samples were treated with osmium tetroxide and were analyzed with a Multisizer Coulter. Cell size distributions were computed for each sample with exponential and Gaussian function fits. RESULTS: Computed tomography-measured visceral fat accumulation was the best predictor of larger cell populations as well as the percentage of small cells in both OM and SC fat (P<0.0001 for all). Accordingly, women with visceral obesity had larger cells in the main population and higher proportion of small adipocytes independent of total adiposity (P≤0.05). Using linear regression analysis, we found that women characterized by larger-than-predicted adipocytes in either OM or SC adipose tissue presented higher visceral adipose tissue area, increased percentage of small cells and homeostasis model assessment insulin resistance index as well as higher OM adipocyte isoproterenol-, forskolin- and dbcAMP-stimulated lipolysis compared to women with smaller-than-predicted adipocytes, independent of total adiposity (P≤0.05). CONCLUSION: Excess visceral adipose tissue accumulation is a strong marker of both adipocyte hypertrophy and increased number of small cells in either fat compartment, which relates to higher insulin resistance index and lipolytic response, independent of total adiposity.
BACKGROUND:Visceral obesity is independently related to numerous cardiometabolic alterations, with adipose tissue dysfunction as a central feature. OBJECTIVE: To examine whether omental (OM) and subcutaneous (SC) adipocyte size populations in women relate to visceral obesity, cardiometabolic risk factors and adipocyte lipolysis independent of total adiposity. DESIGN AND METHODS: OM and SC fat samples were obtained during gynecological surgery in 60 women (mean age, 46.1±5.9 years; mean BMI, 27.1±4.5 kg/m² (range, 20.3-41. kg/m²)). Fresh samples were treated with osmium tetroxide and were analyzed with a Multisizer Coulter. Cell size distributions were computed for each sample with exponential and Gaussian function fits. RESULTS: Computed tomography-measured visceral fat accumulation was the best predictor of larger cell populations as well as the percentage of small cells in both OM and SC fat (P<0.0001 for all). Accordingly, women with visceral obesity had larger cells in the main population and higher proportion of small adipocytes independent of total adiposity (P≤0.05). Using linear regression analysis, we found that women characterized by larger-than-predicted adipocytes in either OM or SC adipose tissue presented higher visceral adipose tissue area, increased percentage of small cells and homeostasis model assessment insulin resistance index as well as higher OM adipocyte isoproterenol-, forskolin- and dbcAMP-stimulated lipolysis compared to women with smaller-than-predicted adipocytes, independent of total adiposity (P≤0.05). CONCLUSION: Excess visceral adipose tissue accumulation is a strong marker of both adipocyte hypertrophy and increased number of small cells in either fat compartment, which relates to higher insulin resistance index and lipolytic response, independent of total adiposity.
Authors: Ursula A White; Mark D Fitch; Robbie A Beyl; Marc K Hellerstein; Eric Ravussin Journal: J Clin Endocrinol Metab Date: 2017-07-01 Impact factor: 5.958
Authors: Elisa Belluzzi; Veronica Macchi; Chiara Giulia Fontanella; Emanuele Luigi Carniel; Eleonora Olivotto; Giuseppe Filardo; Gloria Sarasin; Andrea Porzionato; Marnie Granzotto; Assunta Pozzuoli; Antonio Berizzi; Manuela Scioni; Raffaele De Caro; Pietro Ruggieri; Roberto Vettor; Roberta Ramonda; Marco Rossato; Marta Favero Journal: Int J Mol Sci Date: 2020-08-21 Impact factor: 5.923
Authors: Hamish M Aitken-Buck; Mohammed Moharram; Aram A Babakr; Robin Reijers; Isabelle Van Hout; Ingrid C Fomison-Nurse; Ramanen Sugunesegran; Krishna Bhagwat; Phillip J Davis; Richard W Bunton; Michael J A Williams; Martin K Stiles; Peter P Jones; Sean Coffey; Regis R Lamberts Journal: Adipocyte Date: 2019-12 Impact factor: 4.534