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Literature DB >> 2657660

Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants.

D M Woodcock¹, P J Crowther, J Doherty, S Jefferson, E DeCruz, M Noyer-Weidner, S S Smith, M Z Michael, M W Graham.

Abstract

Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage and plasmid cloning systems. Quantitative estimations of relative tolerance to methylation for these strains are presented, together with the evaluation of the most promising strains in practical recombinant cloning situations. Host strains are recommended for different recombinant cloning requirements. These data also provide a rational basis for future construction of 'ideal' hosts combining optimal methylation tolerance with additional advantageous mutations.

Entities: Chemical Species

Mesh：

Substances：
Cytosine

Year: 1989 PMID： 2657660 PMCID： PMC317789 DOI： 10.1093/nar/17.9.3469

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

19 in total

1. Restriction and modification in vivo by Escherichia coli K12.

Authors: E A Raleigh
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

2. Host strains that alleviate underrepresentation of specific sequences: overview.

Authors: A R Wyman; K F Wertman
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

3. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

Authors: W B Wood
Journal: J Mol Biol Date: 1966-03 Impact factor: 5.469

4. Molecular cloning and identification of protein products of rglB locus of Escherichia coli.

Authors: S Sozhamannan; K Dharmalingam
Journal: Gene Date: 1988-12-25 Impact factor: 3.688

5. The hsd (host specificity) genes of E. coli K 12.

Authors: B Sain; N E Murray
Journal: Mol Gen Genet Date: 1980

6. KpnI families of long, interspersed repetitive DNAs associated with the human beta-globin gene cluster.

Authors: B Shafit-Zagardo; F L Brown; J J Maio; J W Adams
Journal: Gene Date: 1982-12 Impact factor: 3.688

7. Site-specific methylases induce the SOS DNA repair response in Escherichia coli.

Authors: J Heitman; P Model
Journal: J Bacteriol Date: 1987-07 Impact factor: 3.490

8. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.

Authors: E A Raleigh; G Wilson
Journal: Proc Natl Acad Sci U S A Date: 1986-12 Impact factor: 11.205

9. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB.

Authors: M Noyer-Weidner; R Diaz; L Reiners
Journal: Mol Gen Genet Date: 1986-12

10. Human DNA (cytosine-5)methyltransferase selectively methylates duplex DNA containing mispairs.

Authors: S S Smith; T A Hardy; D J Baker
Journal: Nucleic Acids Res Date: 1987-09-11 Impact factor: 16.971

322 in total

1. Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli.

Authors: M Blokesch; A Magalon; A Böck
Journal: J Bacteriol Date: 2001-05 Impact factor: 3.490

Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants.

1. Restriction and modification in vivo by Escherichia coli K12.

2. Host strains that alleviate underrepresentation of specific sequences: overview.

3. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

4. Molecular cloning and identification of protein products of rglB locus of Escherichia coli.

5. The hsd (host specificity) genes of E. coli K 12.

6. KpnI families of long, interspersed repetitive DNAs associated with the human beta-globin gene cluster.

7. Site-specific methylases induce the SOS DNA repair response in Escherichia coli.

8. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.

9. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB.

10. Human DNA (cytosine-5)methyltransferase selectively methylates duplex DNA containing mispairs.

1. Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli.

2. Sinorhizobium meliloti plasmid pRm1132f replicates by a rolling-circle mechanism.

3. Novel group of virulence activators within the AraC family that are not restricted to upstream binding sites.

4. DNA translocation blockage, a general mechanism of cleavage site selection by type I restriction enzymes.

5. Characterization of SepL of enterohemorrhagic Escherichia coli.

Review 6. Structure and function of pectic enzymes: virulence factors of plant pathogens.

7. Novel genomic rearrangement that affects expression of the Streptococcus pyogenes streptolysin O (slo) gene.

8. The function of SECIS RNA in translational control of gene expression in Escherichia coli.

9. Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins.

10. LasR, a transcriptional activator of Pseudomonas aeruginosa virulence genes, functions as a multimer.