F C Santos1, C D Corcini2, V G Costa2, S M Gheller3, C E Nogueira4, B da Rosa Curcio4, A S Varel5. 1. Departamento de Clinicas Veterinarias, Faculdade de Veterinaria, Universidade; Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Federal de Pelotas, Campus Universitario, Pelotas, Brasil. antoniovarela@furg.br and carlini@portoweb.com.br. 2. Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Federal de Pelotas, Campus Universitario, Pelotas, Brasil. 3. Departamento de Patologia Animal, Laboratorio de Reproduçao Animal, Federal de Pelotas, Campus Universitario, Pelotas, Brasil. 4. Departamento de Clinicas Veterinarias, Faculdade de Veterinaria, Universidade, Federal de Pelotas, Campus Universitario, Pelotas, Brasil. 5. Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Faculdade de Veterinaria, Universidade, Federal de Pelotas; Reproduçao Animal Comparada, Instituto de Ciencia Biologicas, Universidade Federal de Rio Grande, Rio Grande, Brasil.
Abstract
BACKGROUND: Solid storage medium prevents cellular sedimentation, reduces metabolic demand via limiting movement, and avoids the modification of an extender composition in the sedimentary microenvironment. It has been proven to prolong spermatozoa viability in mammalians. OBJECTIVE: This experiment aims to evaluate the effect of cool storage in solid phase extender on stallion sperms. MATERIALS AND METHODS: Semen was collected from 10 Crioulo stallions (n=30) and submitted to treatments: control group (semen extender) and groups with gelatin addition in different concentrations (semen extender + 1%, 2% and 3%). Seminal analyses included motility, mitochondrial functionality, plasma membrane integrity, DNA and acrosome at 0; 24; 48 and 72 hours during cooled storage at 5 degree C. RESULTS: Motility, mitochondrial functionality, plasma membrane and acrosome integrity declined during storage time, with no statistical difference between treatments. DNA integrity did not significantly change during storage period. CONCLUSION: Solid medium was not harmful and did not improved stallion sperm parameters during cooled storage.
BACKGROUND:Solid storage medium prevents cellular sedimentation, reduces metabolic demand via limiting movement, and avoids the modification of an extender composition in the sedimentary microenvironment. It has been proven to prolong spermatozoa viability in mammalians. OBJECTIVE: This experiment aims to evaluate the effect of cool storage in solid phase extender on stallion sperms. MATERIALS AND METHODS: Semen was collected from 10 Crioulo stallions (n=30) and submitted to treatments: control group (semen extender) and groups with gelatin addition in different concentrations (semen extender + 1%, 2% and 3%). Seminal analyses included motility, mitochondrial functionality, plasma membrane integrity, DNA and acrosome at 0; 24; 48 and 72 hours during cooled storage at 5 degree C. RESULTS: Motility, mitochondrial functionality, plasma membrane and acrosome integrity declined during storage time, with no statistical difference between treatments. DNA integrity did not significantly change during storage period. CONCLUSION:Solid medium was not harmful and did not improved stallion sperm parameters during cooled storage.