H Ma1, D Liu2, W Wang3, L Wang3, Bo Fu3, Z Li3, X He3. 1. Heilongjiang Academy of Agricultural Sciences, Harbin, China. mahong_ok@163.com. 2. Heilongjiang Academy of Agricultural Sciences, Harbin, China. m18104626703_1@163.com. 3. Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Abstract
BACKGROUND: Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource. OBJECTIVE: To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig. MATERIALS AND METHODS: Ejaculated min pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated. RESULTS: The quality of post-thawed sperm samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25 mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05). CONCLUSION: Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
BACKGROUND: Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource. OBJECTIVE: To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig. MATERIALS AND METHODS: Ejaculated min pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated. RESULTS: The quality of post-thawed sperm samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25 mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05). CONCLUSION:Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.